Figure 6
Evaluation of the immune response after bacterial infection. (A) The cellular components of the hemolymph are granulocytes and hyalinocytes. Photographs were taken using Nomarski DIC objective. (B) Apoptotic and necrotic levels induced by V. alginolyticus in primary cultures of hemocytes (n = 6). (C) In vivo modulation of the cellular concentration in hemolymph after bacterial infection. A significant increment in the number of cells was observed 6 h after infection. Graph represents the measurement of 6 independent animals as well as the mean + SEM. (D) Apoptotic and necrotic levels in vivo induced by V. alginolyticus at different times post infection. In all graphs, bars represent the mean + SEM of 6 independent measurements. Data were analyzed using the Student’s t-test. (*) significant differences at p < 0.05.