Table 4 Primers designed for the microarray validation
Primer name | Sequence 5′ → 3′ | Tm | Product size | Ct slope | Acc. n°/Probe name |
|---|---|---|---|---|---|
Clam 18S F | CCGAACATCTAAGGGCATCA | 60.12 | 169 bp | −3.0 | EF426293.1 |
Clam 18S R | AGTTGGTGGAGCGATTTGTC | 60.99 | |||
C3 F | CCCAGGTGCCAAAGAACA | 55.56 | 162 bp | −3.5 | S_isotig17547_isogroup06014 |
C3 R | GCGGGGTACACATACTCGTC | 60.00 | |||
IL-17D F | CTCAAAAAGACTCACAGGGAATG | 60.15 | 186 bp | −3.8 | P_isotig09595_isogroup01099 |
IL-17D R | CTGGCAATGATGTACTGTCGTAA | 60.07 | |||
LTBP-4 F | TAATCATTGCCGCCTTATCG | 60.92 | 188 bp | −3.6 | S_isotig21547_isogroup10014 |
LTBP-4 R | GCGACCTGAATCAAATTCGT | 60.08 | |||
TRAF6 F | GCCAACATAGTAGCTCAGGAACA | 60.68 | 148 bp | −3.4 | P_isotig17929_isogroup06396 |
TRAF6 R | TTCCAATATAGCTTACAACATCAACA | 59.08 |