From: BisQC: an operational pipeline for multiplexed bisulfite sequencing
Ā | Raw-input | Aligned input | Unique aligned | % Unique aligned | 1X COV (# of CpGs) | 5X COV (# of CpGs) | 10X COV (# of CpGs) | Mean COV | |
---|---|---|---|---|---|---|---|---|---|
Lane 1 1% non bis-converted DNA | B02 | 33 064 288 | 2 855 963 | 2 010 850 | 6.08 | 2 567 012 | 349 168 | 40 326 | 2.63 |
D07 | 22 138 393 | 1 819 725 | 1 255 728 | 5.67 | 2 062 303 | 121 179 | 6 308 | 1.98 | |
E08 | 27 604 275 | 3 277 855 | 2 323 092 | 8.41 | 2 566 936 | 527 206 | 109 576 | 3.27 | |
G12 | 34 606 411 | 3 331 718 | 2 318 284 | 6.69 | 2 714 010 | 422 645 | 47 241 | 2.81 | |
Lane 2 10% non bis-converted DNA | B02 | 35 305 177 | 2 951 835 | 2 078 732 | 5.89 | 2 567 438 | 380 661 | 48 125 | 2.73 |
D07 | 13 678 215 | 1 406 910 | 999 858 | 7.31 | 1 870 722 | 63 063 | 2 673 | 1.77 | |
E08 | 29 182 244 | 3 343 563 | 2 371 936 | 8.13 | 2 564 006 | 549 654 | 119 076 | 3.35 | |
G12 | 35 675 527 | 3 377 167 | 2 352 532 | 6.59 | 2 706 283 | 443 966 | 50 919 | 2.87 | |
Lane 3 20% non bis-converted DNA | B02 | 29 189 642 | 2 680 090 | 1 903 402 | 6.52 | 2 506 260 | 315 769 | 33 092 | 2.55 |
D07 | 11 434 385 | 1 281 232 | 1 281 232 | 11.2 | 1 798 599 | 47 005 | 1 960 | 1.69 | |
E08 | 29 178 605 | 3 315 119 | 2 354 427 | 8.07 | 2 555 851 | 543 668 | 117 267 | 3.33 | |
G12 | 32 077 566 | 3 190 847 | 2 234 841 | 6.97 | 2 672 851 | 396 964 | 41 721 | 2.75 | |
Lane 4 30% non bis-converted DNA | B02 | 26 075 222 | 2 601 566 | 1 853 844 | 7.11 | 2 471 169 | 302 050 | 30 356 | 2.52 |
D07 | 15 915 092 | 1 563 145 | 1 101 459 | 6.92 | 1 923 817 | 86 567 | 3 720 | 1.87 | |
E08 | 22 095 982 | 2 896 518 | 2 075 605 | 9.39 | 2 456 950 | 446 280 | 80 977 | 3.04 | |
G12 | 27 526 678 | 3 034 738 | 2 139 571 | 7.77 | 2 629 556 | 363 919 | 35 701 | 2.68 | |
Lane 5 50% non bis-converted DNA | B02 | 20 542 906 | 2 606 323 | 1 841 922 | 8.97 | 2 368 596 | 321 222 | 32 684 | 2.62 |
D07 | 9 506 379 | 1 299 321 | 925 451 | 9.74 | 1 716 465 | 57 531 | 2 107 | 1.78 | |
E08 | 18 652 337 | 2 828 208 | 2 007 876 | 10.76 | 2 359 943 | 440 954 | 81 427 | 3.07 | |
G12 | 23 046 688 | 3 069 911 | 2 146 208 | 9.31 | 2 559 434 | 391 118 | 36 982 | 2.77 | |
Lanes 6 20% non bis-converted DNA | B02 | Ā | Ā | Ā | Ā | - | Ā | Ā | Ā |
D07 | Ā | Ā | Ā | Ā | - | Ā | Ā | Ā | |
E08 | Ā | Ā | Ā | Ā | - | Ā | Ā | Ā | |
G12 | 147 537 115 | 6 665 204 | 4 381 541 | 2.97 | 2 988 624 | 1 086 692 | 383 384 | 4.93 | |
Lane 7 20% non bis-converted DNA | B02 | 20 450 646 | 2 241 773 | 1 614 966 | 7.9 | 2 395 119 | 213 017 | 15 790 | 2.26 |
D07 | 8 334 712 | 1 087 639 | 791 533 | 9.5 | 1 671 805 | 27 718 | 1 338 | 1.57 | |
E08 | 19 548 711 | 2 663 849 | 1 915 409 | 9.8 | 2 415 658 | 385 375 | 62 823 | 2.85 | |
G12 | 21 853 857 | 2 654 189 | 1 886 840 | 8.63 | 2 562 616 | 267 113 | 20 703 | 2.42 | |
Lane 8 20% non bis-converted DNA | B02 | 10 420 846 | 1 644 906 | 1 213 820 | 11.65 | 2 156 458 | 95 891 | 4 665 | 1.89 |
D07 | 4 061 039 | 774 145 | 578 180 | 14.24 | 1 394 239 | 8 729 | 914 | 1.39 | |
E08 | 6 950 628 | 1 494 036 | 1 105 554 | 15.91 | 1 994 770 | 113 007 | 9 195 | 1.98 | |
G12 | 11 162 134 | 1 928 010 | 1 405 997 | 12.6 | 2 334 809 | 120 111 | 6 110 | 1.98 |