Deep sequencing of the tobacco mitochondrial transcriptome reveals expressed ORFs and numerous editing sites outside coding regions
© Grimes et al.; licensee BioMed Central Ltd. 2014
Received: 16 September 2013
Accepted: 13 January 2014
Published: 17 January 2014
The purpose of this study was to sequence and assemble the tobacco mitochondrial transcriptome and obtain a genomic-level view of steady-state RNA abundance. Plant mitochondrial genomes have a small number of protein coding genes with large and variably sized intergenic spaces. In the tobacco mitogenome these intergenic spaces contain numerous open reading frames (ORFs) with no clear function.
The assembled transcriptome revealed distinct monocistronic and polycistronic transcripts along with large intergenic spaces with little to no detectable RNA. Eighteen of the 117 ORFs were found to have steady-state RNA amounts above background in both deep-sequencing and qRT-PCR experiments and ten of those were found to be polysome associated. In addition, the assembled transcriptome enabled a full mitogenome screen of RNA C→U editing sites. Six hundred and thirty five potential edits were found with 557 occurring within protein-coding genes, five in tRNA genes, and 73 in non-coding regions. These sites were found in every protein-coding transcript in the tobacco mitogenome.
These results suggest that a small number of the ORFs within the tobacco mitogenome may produce functional proteins and that RNA editing occurs in coding and non-coding regions of mitochondrial transcripts.
KeywordsMitochondrial transcriptome Plant mitogenome Tobacco Nicotiana tabacum RNA editing
Angiosperm mitochondrial genomes range from 200,000 to more than 2.6 million bp. These large size differences are due to highly variable intergenic regions that lie between a relatively conserved set of protein coding genes[1, 2]. Inter-species comparisons of mitogenomes suggest they undergo frequent inter- and intra-molecular recombination and tend to acquire both chloroplast and nuclear genetic material. In addition, short degenerate repeats are common between genes in cucurbit mtDNA. Another contributing force to the chimeric nature of plant mitochondrial genomes is their ability to readily uptake DNA through horizontal gene transfer. Richardson and Palmer showed that the mitochondria of the dicot Amborella trichopoda contained sequences homologous to different species’ mitogenomes. With their highly recombinant DNA, propensity for genomic double strand breakage, and perpetual ability to undergo fusion and fission, these organelles set themselves apart from the rest of the cell regarding potential for genomic diversity.
The frequent recombination and transfer events have not only expanded the intergenic regions, but also produce possible protein-coding open reading frames (ORFs) in some species. Small ORFs can comprise a significant amount of the mitogenome; for example there are 117 poorly characterized small ORFs in the tobacco mitogenome, compared to 60 genes with identifiable functions. Almost all of these are uncharacterized in tobacco, but some homologous sequences have been linked to cytoplasmic male sterility (CMS) in other species[7, 8]. Orfs 25 and 265 in sorghum have been shown to control CMS and are conserved among the mitogenomes of Oryza and Triticum[10, 11]. These mitogenomic ORFs may also indirectly modulate RNA steady-state levels since cytoplasmic message background affects RNA degradation.
The plant mitogenome is transcribed by phage-type (T7 and T3-like) nuclear-encoded RNA polymerase (RNAP). In eudicots, two RNAPs localize to mitochondria: RpoTm, which exclusively localizes to the mitochondria, and RpoTmp, which localizes to both mitochondria and plastids. RpoTm is probably the primary polymerase and RpoTmp transcribes mitochondrial genes early in development. Plant mitochondrial genes often possess multiple promoters consisting of core tetranucleotides CRTA, ATTA, and RGTA that are part of a nonanucleotide conserved sequence, CRTAaGaGA, and an AT-rich region upstream from the start site[15, 16]. As more mitogenomic data has become available, genes without obvious promoter motifs and possible promoter sequences within intergenic regions have been discovered. There are two different descriptions of plant mitochondrial transcription that are linked to polymerase type. Kuhn et al. have shown that RpoTmp is gene specific rather than promoter specific. This opens the possibility of cis-acting elements specifically directing transcription. Other studies have observed non-specific transcription of the intergenic regions resulting in large quantities of “junk” transcripts[18–20]. This finding, coupled with an observation of loosely controlled transcription termination—which produces long run-on RNAs—suggests indiscriminate low-scale expression of much of the mitogenome, most likely by the RpoTm polymerase[21, 22].
These long transcripts undergo a series of processing events to produce functional transcripts[23, 24]. Transcript editing is a ubiquitous and widely encountered processing event in plant mitochondria. Every protein-coding sequence in a mitogenome is likely to be edited; overall editing in angiosperms are estimated to occur at about 500 sites per genome with a range from 189 in Silene noctiflora, to 600 in date palm. Almost all mitochondrial editing is performed through the process of cytosine to uracil conversion (C→U). Editing has been linked to generating start and stop codons, enabling protein function by altering amino acid content, and restoring fertility in cases of cytoplasmic male sterility (CMS)[28–30]. Lu and Hanson demonstrated that protein products from the atp6 gene in Petunia were made exclusively from completely edited transcripts within the mitochondria. Alternately, polypeptides from unedited or partially edited transcripts accumulate in Zea mays. The consequences of this are still under investigation, but from a gene regulation perspective, partially edited transcripts can potentially provide a variety of gene products from a single coding region.
In this study, deep sequencing was used to assemble the tobacco mitochondrial transcriptome. This enabled the determination of mono- and polycistronic transcripts, identification of expressed uncharacterized ORFs, and a whole transcriptome level estimate of editing sites. We found nine monocistronic and sixteen polycistronic transcripts. Eighteen uncharacterized ORFs were transcribed, eleven of which were found to be polysome associated. Six hundred and thirty five potential edits were found with 562 occurring within protein coding genes.
Deep sequencing and alignment of the tobacco mitochondrial transcriptome
Poly- and mono-cistronic transcripts as predicted from the tobacco mitogenome transcriptome assembly
Two genes, cox1 and atp6, exhibited a precipitous drop of DOC in the middle of these coding regions. These were considered possible uncharacterized transcript processing events so end-point PCR and RT-PCR were performed on DNA and RNA, respectively. Both PCR and RT-PCR reactions yielded amplicons of equal size, consistent with the published genome annotation (data not shown). This suggests the low DOC in these two genes does not indicate a processing event but is instead a technical inconsistency
RNA edit sites
Potential C→U edit sites were identified in the transcriptome assembly by comparing RNA reads to the published mitogenome sequences (GenBank accessions NC_006581.1 and BA000042). Edit sites were chosen if the DOC was >200 and the RNA edit percentage less than 100%; nucleotides with a 100% change rate between RNA and the genome sequence were considered SNPs. This methodology identified 540C→U edit sites across the entire mitogenome (Additional file3: Table S2). When compared to previously identified edit sites (PIES), this methodology failed to recognize 95 PIES but found 119 potential new sites. Combined, PIES and new sites equaled 635 total edit sites. A supermajority of the sites, 573, were in protein-coding regions and included every identifiable protein-encoding transcript plus five transcribed orfs. Only two exons, rpl2_exon1 and rps3_exon1, did not have potential edits. Among the 119 newly identified edit sites, 41 were found in coding regions, 5 in tRNAs, and 73 in intergenic regions. Forty three of the intergenic edits were in 5′ and 3′ UTRs, 23 in intergenic regions of polycistronic transcripts, and 7 in regions that were not coding regions or linked to any identifiable transcript (Additional file4: Table S3).
ORF steady state RNA abundance analysis
List of open reading frames chosen for this study
Genome start site
Genome stop site
Number of edit sites
Protein homologies of Polysome associated ORFs
full length ATPase subunit 8
full length ATPase subunit 4
fragment of ribosomal protein S1
full length ribosomal protein L10
fragment of cytochrome coxdase
full length ribosomal protein L10
Deep-sequencing results were confirmed with qRT-PCR analysis of three biological replicates (two technical replicates from each biological for a total of n = 6) and Mann–Whitney non-parametric analysis was used to determine significant differences. For all qRT-PCR experiments, the cox2 mitochondrial protein-coding gene was used as a positive control and for normalization. Background was measured from the orf161 region, which had a DOC below 75 and qRT-PCR copy number estimates well below transcribed regions.
Polysome analysis of open reading frames
All the confirmed transcribed ORFs were subjected to polysomal analysis to test for evidence of translation. Cox2 was used as a positive control and orf161 to measure background. Cox2 was also quantified in EDTA-treated extracts as a negative control. RNA in polysomal pellets and supernatants from three biological replicates were purified and measured by qRT-PCR and Mann–Whitney non-parametric analysis was used to determine significant differences. Orfs177, 265/atp8, 25/atp4, 222, 216, 147, 160, 115, 166b, and 159b/rpl10 were found in polysomal pellets at significantly higher amounts than background (Table 2 and Additional file6: Table S5). All but one (orf175) was successfully detected in the supernatant at levels significantly higher than background.
Translations of the eleven polysome-associated ORFs were screened using GenBank (http://www.ncbi.nlm.nih.gov) and Sol Genomics Network (http://solgenomics.net) to see if any encode identifiable proteins found in other mitochondrial genomes. Orfs 147 and 159b encode putative full-length RPL10 proteins, orf216 encodes a full-length mitochondrial rps1, orf25 encodes a full-length atp4 coding region, and orf265b is atp8. Orfs160, 115, and 166b do not encode identifiable proteins. Orfs177 and 222 match uncharacterized nuclear genes from Nicotiana benthamiana. Ten of the ORFs were found in the mitochondrial genomes of other genera; only orf222 was unique to Nicotiana.
The tobacco mitochondrial transcriptome
Deep sequencing of the tobacco mitochondrial transcriptome detected multiple mono- and polycistronic transcripts with relatively long 3′ and 5′ UTRs. The most highly transcribed regions contained protein-coding regions, and the length and content of the transcripts suggested extensive post-transcriptional processing takes place. It has been suggested that mitochondrial genes between 3,000 and 8,000 nucleotides apart will most likely be transcribed as a cluster which are then processed and edited to form translatable mRNAs. Pre-mRNA maturation takes place in the matrix with 5′ end modification occurring through endonuclease activity or, at times, the pre-mRNA strand does not need 5′ processing; the 5′ end is simply the beginning of the reading frame. The 3′ processing is much more nebulous in nature, whereas some of the transcripts have been proposed to be terminated with the help of mitochondrial transcription termination factors (mTERFs), other strands simply run on well past the end of the reading frame— the latter situation suggests that transcription termination in plant mitochondria is not critically important[36, 37]. Nevertheless, the 3′ end is still important in protecting the RNA from exonuclease activity, as local cis-acting elements aid in step loop formation.
In our analysis, intron and exon steady state abundance were generally distinguishable with exonic DOC much higher than intronic, suggesting that introns are degraded after removal. The intergenic regions were mostly non/low-transcribed, although there were some regions with high DOC. Some of these had ORFs predicted in the initial tobacco mitogenome annotation.
Our observations confirm that long mitochondrial transcripts are produced, but these transcripts primarily contained protein-coding regions. More than 57% of the transcriptome had a DOC below 150 and protein-coding regions were clearly distinguishable from intergenic spaces. This suggests transcription was focused on protein-coding regions and that transcription is only occurring at high rates in specific areas. Plant mitochondrial gene expression is portrayed as a relaxed and inefficient coordination of two phage-type RNA polymerases (RpoTm and RpoTmp). This is based on the placement of promoters that have been found upstream of protein coding genes as well as scattered throughout intergenic regions. The overall result is long run-on transcripts found throughout plant mitochondrial transcriptomes and large quantities of cryptic transcripts from intergenic regions[36, 37]. Alternatively, mitochondrial transcription rates have been shown to vary considerably between different genes – most likely due to different promoter strengths and unique stoichiometric needs of each gene.
Mitogenome edit sites
Transcriptome analysis revealed 540C→U edits in the tobacco mitochondrial transcriptome which, if combined with PIES, gives 635 total edit sites with 562 in coding regions. This observation is consistent with estimates from other higher plant mitochondria, where an average of 500 edit sites per mitogenome has been suggested, with most of those edits occurring in coding regions. In our analysis, transcriptome assembly accompanied by the SNP function in the assembly package failed to recognize 95 of the previously reported sites but identified 119 edit sites unreported in previous analyses (GenBank accession BA000042 and).
The sites missed in the transcriptome analysis were manually inspected and all appear to be edited according to the alignment, although some at very low rates (<10%). We suspect these sites were not identified by the SNP function because they were in areas with erroneously low mid-transcript DOC’s and/or the percentage of edited nucleotides was much lower than expected based on other sites (<50%).
Most of the 119 newly identified edit sites were not in coding regions revealing a larger proportion of intergenic edit sites than identified in previous screens. Very few instances of editing in non-coding regions exist. In Arabidopsis thaliana 15 of 456 sites are reported outside of coding regions and in tobacco, one site had been identified (NC_006581.1 and). A definitive reason to edit non-coding regions is elusive, but some have been linked to splicing[41, 42]. Most of the non-coding region editing sites found in this study were in UTR regions suggesting they may be necessary for processing or translation. Others found in intron regions could be a prerequisite for splicing. It is also possible that some of these are unnecessary and indicate superfluous editing similar to the silent editing reported in some third codon positions.
Expression of mitogenomic open reading frames
In this study, 18 uncharacterized open reading frames were found in transcribed regions and then confirmed with qRT-PCR. Polysome analysis showed 10 transcripts from those ORF’s were attached to ribosomes. Two, orfs25 and 265b are homologous to atp4 and 8, have been linked to cytoplasmic male sterility in sorghum, and are found in at least two other mitogenomes[10, 11]. Three others encode putative full-length proteins. Orf159b was recently characterized as rpl10 in angiosperm mitochondria, including N. tabacum. Orf147 also appears to encode a near full length RPL10 protein. The role of orf147 as a second truncated rpl10 is unknown. Orf216 encodes a mitochondrial RPS1 protein. RPS1 has not been defined in tobacco, but has been identified in the mitogenome other plants such as wheat and primrose[45, 46]. Five ORFS —160, 115, 166b, 177, and 222 do not encode identifiable proteins but are transcribed and polysome associated.
There are conflicting hypotheses regarding the possible benefit mitochondrial open reading frames provide. Some suggest their origin through recombination events create a burden on the organelle’s RNA processing systems which would suggest selection against their presence. Beaudet et al. found some mitogenomic ORFs were mobile elements specializing in horizontal gene transfer; these were responsible for making chimeric versions of existing mitochondrial genes suggesting they benefit the organelle. Our data suggests they are not merely present but some are also transcribed and possibly translated.
Transcriptome analysis of the tobacco mitogenome demonstrated that the chromosome is transcribed as discrete mono- and poly-cistronic transcripts with low- or non-transcribed intervening sequences. This confirms previous observations and suggestions of multiple promoter sites throughout the mitogenome. An inventory of RNA edit sites shows that widespread editing is not limited to coding regions in the tobacco mito-transcriptome. This suggests editing enzymes do not discriminate between coding and non-coding RNA. Some plant mitochondrial genomes have numerous uncharacterized ORFs that may be functional genes or recombinational remnants. The data presented in this study show that 18 of the 117 poorly characterized ORFs in the tobacco mitogenome are transcribed and 10 are polysome associated. This suggests that some of these produce functional proteins.
Plant material and RNA extraction
Nicotiana tabacum, var. petit Havana was used for all experiments. For leaf tissue, 2–3 cm specimens were removed from plants grown in commercial potting soil in a Percival PGC-10 incubator set for a 16 hour day/8 hour night cycle at 28°C. Root tissues were dissected from 3 three-week- old seedlings grown from surface sterilized seeds. Briefly, tobacco seeds were sterilized by washing briefly with 70% ethanol for 30 seconds followed by a 50% bleach solution for 15 minutes with agitation. Seeds were rinsed three times with sterile water and transferred to sterile Magenta boxes. Boxes were prepared by placing several layers of 2′x 2′ paper towel squares wetted with a 1/3X concentration of Miracle Gro liquid fertilizer in the bottom and autoclaving. Root tissue was excised from healthy tobacco plants using a surgical blade and dissection microscope. Flowers were removed from the same set of plants that were the source of leaf tissue. They were collected when the corolla was fully open and shedding pollen.
For RNA extraction, tissues were frozen in liquid nitrogen and ground into a powder using a mortar and pestle. RNA was extracted with Qiagen’s RNeasy kit with the additional DNAse steps (Hercules, CA) following the manufacturer’s instructions. RNA concentrations were measured using a NanoDrop Lite (Thermo Scientific, Waltham, MA).
Deep sequencing of the tobacco mitochondrial transcriptome
Total tobacco RNA was prepared at MTSU and sent to the University of Illinois sequencing center (Springfield, IL) for transcriptome sequencing. Ribosomal RNAs were removed from the total RNA using Ribo-Zero (Epicentre, Madison, WI) and a total RNA library prepared using Illumina’s TruSeq RNAseq Sample Prep kit (San Diego, CA). Libraries were sequenced on one lane for 100 cycles from each end on an Illumia HiSeq2000 platform using a TruSeq SBS sequencing kit v.3 and analyzed with Casava 1.8. 163,836,382 sequence reads were reported with an average length of 100nt. Once sequence data was received by the team at MTSU, sequences were aligned to the tobacco mitochondrial genome (Genbank NC_006581) using DNAstar’s (Madison, WI) Seqman NGen program to produce a depth-of-coverage map.
Polysomal RNA isolation and purification for translation analysis
Polysomal RNA was extracted and isolated using a protocol modified from Mayfield et al.. 200–400 mg of leaf tissue was harvested and ground into a powder with liquid nitrogen. The frozen powder was quickly re-suspended in extraction buffer (200 mM Tris HCL, pH 9.0, 200 mM KCL, 35 mM MgCl2, 25 mM EGTA, 200 mM sucrose, 1% Triton-X 100, 2% BRIJ, 0.5 mg/ml heparin, 0.7% 2-mercaptoethanol, and 100 mg/ml chloramphenicol). Cell debris was removed by centrifugation at 17,000xg for 10 min at 4°C, supernatant transferred to a new tube, and deoxycholate added to a final concentration of 0.05%. Samples were centrifuged at 13,000xg for 10 min at 4°C. The remaining supernatant was then layered onto a two-step sucrose gradient (1.75 M and 0.5 M sucrose in 1x cushion buffer: 40 mM Tris HCL, pH 9.0, 200 mM KCL, 30 mM MgCL2, 5 mM EGTA, 0.5 mg/ml heparin, 0.7% 2-mercaptoethanol, and 100 mg/ml chloramphenicol). Polysomal RNA was pelleted by ultracentrifugation at 180,000xg for 120 min at 4°C. After ultracentrifugation, the supernatant was removed by carefully pipetting and transferring the top layers to new centrifuge tubes on ice. The polysomal RNA was extracted from pellets with Qiagen’s Plant RNeasy Kit following the manufacturer’s protocol. The nucleic acid remaining in the supernatant was precipitated by adding 4 volumes of 95% ethanol, 1/10 volume of sodium acetate, flash frozen in liquid nitrogen, thawed on ice, and pelleted by centrifugation at 30,000xg for 30 min at 4°C. All nucleic acid from polysome analyses was re-precipitated with 3 volumes of 8 M LiCl to remove residual heparin that carried through the extraction process and would inhibit reverse transcriptase.
As a negative control, RNA extracts were incubated with 0.5 M EDTA, vortexed, and placed on ice for 10 min to release ribosomes from transcripts. After incubation on ice, the EDTA/extract mixture was layered on top of sucrose gradients, ultracentrifuged, and processed as described above.
All primer pairs were prepared as described in Sharpe et al.. Briefly, optimal annealing temperatures and PCR efficiencies were empirically determined using a BioRad CFX96 C1000 thermal cycler (Hercules, CA). To ensure the amplification of a single product, melt curves were inspected for each reaction and products of initial reactions were visualized on a 3% agarose gel stained with ethidium bromide. Experimental primers for qRT-PCR were designed and tested for 28 open reading frames, a positive control (cox2), and negative/background control (Orf161) (Additional file7: Table S6). Primers were purchased from Eurofins MWG Operon, Inc. (Huntsville, AL). Optimal annealing temperatures, primer pair efficiencies, and amplicon lengths can be found in Additional file7: Table S6.
qRT-PCR analysis was performed using a Bio-Rad CFX96 C1000 Thermal Cycler (Hercules, CA). PCR reactions were prepared with 5 μl of Quanta PerfeCTa One-Step SYBR Green Mix (Gaithersburg, MD), 2ul 5pmol primers, 50 ng RNA template, 0.1 μl M-MLV reverse transcriptase (Promega, Madison, WI), and nuclease-free water for a 10 μl total reaction volume. Cycle programming started with a 30 min reverse transcriptase step at 45° followed by a 3 min 95° step. The PCR cycle stage was 95°C for 30 Sec 59° for 15 sec and 72°C for 15 sec for 39 cycles. A melt curve was included to ensure the production of single amplicons.
Calculation of estimated copy numbers
Formula 1. N 0 = initial amount of mRNA, F t = fluorescence, E = reaction efficiency, C t = crossover threshold, Amp = amplicon size.
All copy number estimates were normalized against the lowest value of the six cox2 positive control replicates. The normalization factor was calculated by dividing the cox2 copy number estimate from each sample by the smallest cox2 value. This normalization factor was then used to adjust the matching experimental biological and technical replicates across the entire data set. The normalized values were then used to apply standard descriptive statistics such as mean, median, mode, standard deviation, and standard error. The Mann–Whitney rank sum test was used to determine p-values from the normalized copy number data.
Availability of supporting data
The Illumina transcriptome data sets supporting the results presented in this article are available in the National Center for Biotechnology Information – USA (http://www.ncbi.nlm.nih.gov) Sequence Read Archive, accession SRX403934.
The authors wish to thank MTSU’s Molecular Biosciences and Computational Science programs and the College of Graduate Studies for project support. Also included are Rebecca Seipelt-Thiemann (MTSU Biology), Matthew Elrod-Erickson (MTSU Biology), Lisa Bloomer Green (MTSU Mathematics), Erin Etheridge, and two anonymous reviewers for helpful comments, discussions, and manuscript editing.
- Fauron C, Allen JO, Clifton SW, Newton KJ: Plant Mitochondrial Genomes. Molecular Biology and Biotechnology of Plant Organelles. Edited by: Daniell H, Dordrecht CC. 2004, Netherlands: Kluwer Academic Publishers, 151-177.View ArticleGoogle Scholar
- Knoop V, Volkmar U, Hecht J, Grewe F: Mitochondrial Genome Evolution in the Plant Lineage. Plant Mitochondria. Edited by: Kempken F. 2011, New York, NY: Springer, 3-29.View ArticleGoogle Scholar
- Marienfeld J, Unseld M, Brennicke A: The mitochondrial genome of Arabidopsis is composed of both native and immigrant information. Trends Plant Sci. 1999, 4 (12): 495-502. 10.1016/S1360-1385(99)01502-2.PubMedView ArticleGoogle Scholar
- Lilly JW, Havey MJ: Small, repetitive DNAs contribute significantly to the expanded mitochondrial genome of cucumber. Genetics. 2001, 159 (1): 317-328.PubMed CentralPubMedGoogle Scholar
- Richardson AO, Palmer JD: Horizontal gene transfer in plants. J Exper Botany. 2007, 58 (1): 1-9.View ArticleGoogle Scholar
- Sugiyama Y, Watase Y, Nagase M, Makita N, Yagura S, Hirai A, Sugiura M: The complete nucleotide sequence and multipartite organization of the tobacco mitochondrial genome: comparative analysis of mitochondrial genomes in higher plants. Mol Genet Genom MGG. 2005, 272 (6): 603-615. 10.1007/s00438-004-1075-8.View ArticleGoogle Scholar
- Leino M, Landgren M, Glimelius K: Alloplasmic effects on mitochondrial transcriptional activity and RNA turnover result in accumulated transcripts of Arabidopsis ORFs in cytoplasmic male-sterile Brassica napus. Plant J. 2005, 42 (4): 469-480. 10.1111/j.1365-313X.2005.02389.x.PubMedView ArticleGoogle Scholar
- Chang S, Yang T, Du T, Huang Y, Chen J, Yan J, He J, Guan R: Mitochondrial genome sequencing helps show the evolutionary mechanism of mitochondrial genome formation in Brassica. BMC Genom. 2011, 12: 497-10.1186/1471-2164-12-497.View ArticleGoogle Scholar
- Tang HV, Pring DR, Shaw LC, Salazar RA, Muza FR, Yan B, Schertz KF: Transcript processing internal to a mitochondrial open reading frame is correlated with fertility restoration in male-sterile sorghum. Plant J. 1996, 10 (1): 123-133. 10.1046/j.1365-313X.1996.10010123.x.PubMedView ArticleGoogle Scholar
- Akagi H, Sakamoto M, Shinjyo C, Shimada H, Fujimura T: A unique sequence located downstream from the rice mitochondrial atp6 may cause male sterility. Curr Genet. 1994, 25 (1): 52-58. 10.1007/BF00712968.PubMedView ArticleGoogle Scholar
- Rathburn HB, Hedgcoth C: A chimeric open reading frame in the 5′ flanking region of coxI mitochondrial DNA from cytoplasmic male-sterile wheat. Plant Mol Biol. 1991, 16 (5): 909-912. 10.1007/BF00015083.PubMedView ArticleGoogle Scholar
- Liere K, Borner T: Transcription in Plant Mitochondria. Plant Mitochondria. Edited by: Kempken F. 2011, New York, NY: Springer, 85-105.View ArticleGoogle Scholar
- Weihe A: The transcription of plant organelle genomes. Molecular Biology and Biotechnology of Plant Organellles. Edited by: Daniell H, Chase CD. 2004, Dordrecht, Netherlands: Kluwer Academic Publishers, 213-237.View ArticleGoogle Scholar
- Baba K, Schmidt J, Espinosa-Ruiz A, Villarejo A, Shiina T, Gardestrom P, Sane AP, Bhalerao RP: Organellar gene transcription and early seedling development are affected in the rpoT; 2 mutant of Arabidopsis. Plant J. 2004, 38 (1): 38-48. 10.1111/j.1365-313X.2004.02022.x.PubMedView ArticleGoogle Scholar
- Dombrowski S, Binder S: 3′ inverted repeats in plant mitochondrial mRNAs act as processing and stablizing elements but do not terminate transcription. Plant Mitochondria: from Gene to Function. Edited by: Moller IM, Gardestrom P, Glimelius K, Glaser E. 1998, Leiden, Netherlands: Backhuys Publications, 9-12.Google Scholar
- Kuhn K, Weihe A, Borner T: Multiple promoters are a common feature of mitochondrial genes in Arabidopsis. Nucleic Acids Res. 2005, 33 (1): 337-346. 10.1093/nar/gki179.PubMed CentralPubMedView ArticleGoogle Scholar
- Kuhn K, Richter U, Meyer EH, Delannoy E, de Longevialle AF, O’Toole N, Borner T, Millar AH, Small ID, Whelan J: Phage-type RNA polymerase RPOTmp performs gene-specific transcription in mitochondria of Arabidopsis thaliana. Plant Cell. 2009, 21 (9): 2762-2779. 10.1105/tpc.109.068536.PubMed CentralPubMedView ArticleGoogle Scholar
- Perrin R, Lange H, Grienenberger JM, Gagliardi D: AtmtPNPase is required for multiple aspects of the 18S rRNA metabolism in Arabidopsis thaliana mitochondria. Nucleic Acids Res. 2004, 32 (17): 5174-5182. 10.1093/nar/gkh852.PubMed CentralPubMedView ArticleGoogle Scholar
- Holec S, Lange H, Canaday J, Gagliardi D: Coping with cryptic and defective transcripts in plant mitochondria. Biochimica et Biophysica Acta. 2008, 1779 (9): 566-573. 10.1016/j.bbagrm.2008.02.004.PubMedView ArticleGoogle Scholar
- Fang Y, Wu H, Zhang T, Yang M, Yin Y, Pan L, Yu X, Zhang X, Hu S, Al-Mssallem IS, et al: A complete sequence and transcriptomic analyses of date palm (Phoenix dactylifera L.) mitochondrial genome. PloS one. 2012, 7 (5): e37164-10.1371/journal.pone.0037164.PubMed CentralPubMedView ArticleGoogle Scholar
- Dombrowski S, Hoffman M, Kuhn J, Brennicke A, Binder S: On mitochondrial promoters in Arabidopsis thaliana and other flowering plants. Plant Mitochondria: From Gene to Function. Edited by: Moller IM, Gardestrom P, Glimelius K, Glaser E. 1998, Leiden, Netherlands: Backhuys, Publications, 165-170.Google Scholar
- Hoffmann M, Binder S: Functional importance of nucleotide identities within the pea atp9 mitochondrial promoter sequence. J Mol Biol. 2002, 320 (5): 943-950. 10.1016/S0022-2836(02)00552-1.PubMedView ArticleGoogle Scholar
- Lang BF, Laforest MJ, Burger G: Mitochondrial introns: a critical view. Trends Genet TIG. 2007, 23 (3): 119-125. 10.1016/j.tig.2007.01.006.View ArticleGoogle Scholar
- Bonen L: RNA Splicing in Plant Mitochondria. Plant Mitochondria. Edited by: Kempken F. 2011, New York, NY: Springer, 131-155.View ArticleGoogle Scholar
- Bruhs A, Kempken F: RNA Editing in Higher Plant Mitochondria. Plant Mitochondria. Edited by: Kempken F. 2011, New York, NY: Springer, 157-175.View ArticleGoogle Scholar
- Sloan DB, MacQueen AH, Alverson AJ, Palmer JD, Taylor DR: Extensive loss of RNA editing sites in rapidly evolving Silene mitochondrial genomes: selection vs. retroprocessing as the driving force. Genetics. 2010, 185 (4): 1369-1380. 10.1534/genetics.110.118000.PubMed CentralPubMedView ArticleGoogle Scholar
- Takenaka M, Brennicke A: Multiplex single-base extension typing to identify nuclear genes required for RNA editing in plant organelles. Nucleic Acids Res. 2009, 37 (2): e13-PubMed CentralPubMedView ArticleGoogle Scholar
- Hiesel R, Combettes B, Brennicke A: Evidence for RNA editing in mitochondria of all major groups of land plants except the Bryophyta. Proc Natl Acad Sci USA. 1994, 91 (2): 629-633. 10.1073/pnas.91.2.629.PubMed CentralPubMedView ArticleGoogle Scholar
- Gallagher LJ, Betz SK, Chase CD: Mitochondrial RNA editing truncates a chimeric open reading frame associated with S male-sterility in maize. Curr Genet. 2002, 42 (3): 179-184. 10.1007/s00294-002-0344-5.PubMedView ArticleGoogle Scholar
- Kugita M, Yamamoto Y, Fujikawa T, Matsumoto T, Yoshinaga K: RNA editing in hornwort chloroplasts makes more than half the genes functional. Nucleic Acids Res. 2003, 31 (9): 2417-2423. 10.1093/nar/gkg327.PubMed CentralPubMedView ArticleGoogle Scholar
- Lu B, Hanson MR: A single homogeneous form of ATP6 protein accumulates in petunia mitochondria despite the presence of differentially edited atp6 transcripts. Plant Cell. 1994, 6 (12): 1955-1968.PubMed CentralPubMedView ArticleGoogle Scholar
- Phreaner CG, Williams MA, Mulligan RM: Incomplete editing of rps12 transcripts results in the synthesis of polymorphic polypeptides in plant mitochondria. Plant Cell. 1996, 8 (1): 107-117.PubMed CentralPubMedView ArticleGoogle Scholar
- Lohse M, Drechsel O, Kahlau S, Bock R: OrganellarGenomeDRAW-a suite of tools for generating physical maps of plastid and mitochondrial genomes and visualizing expression data sets. Nucleic Acids Res. 2013, 41 (Web Server issue): W575-W581.PubMed CentralPubMedView ArticleGoogle Scholar
- Alvarez MJ, Vila-Ortiz GJ, Salibe MC, Podhajcer OL, Pitossi FJ: Model based analysis of real-time PCR data from DNA binding dye protocols. BMC Bioinform. 2007, 8: 85-10.1186/1471-2105-8-85.View ArticleGoogle Scholar
- Binder S, Holzle A, Jonietz C: RNA Processing and RNA Stability in Plant Mitochondria. Plant Mitochondria. Edited by: Kempken F. 2011, New York, NY: Springer, 107-130.View ArticleGoogle Scholar
- Linder T, Park CB, Asin-Cayuela J, Pellegrini M, Larsson NG, Falkenberg M, Samuelsson T, Gustafsson CM: A family of putative transcription termination factors shared amongst metazoans and plants. Curr Genet. 2005, 48 (4): 265-269. 10.1007/s00294-005-0022-5.PubMedView ArticleGoogle Scholar
- Gagliardi D, Binder S: Expression of the Plant Mitochondrial Genome. Plant Mitochondria. Edited by: Logan D. 2007, Ames, IA: Blackwell Publishing, 50-95.View ArticleGoogle Scholar
- Kuhn J, Tengler U, Binder S: Transcript lifetime is balanced between stabilizing stem-loop structures and degradation-promoting polyadenylation in plant mitochondria. Mol Cell Biol. 2001, 21 (3): 731-742. 10.1128/MCB.21.3.731-742.2001.PubMed CentralPubMedView ArticleGoogle Scholar
- Lenz H, Knoop V: PREPACT 2.0: predicting C-to-U and U-to-C RNA editing in organelle genome sequences with multiple references and curated RNA editing annotation. Bioinform Biol Insights. 2013, 7: 1-19.PubMed CentralPubMedView ArticleGoogle Scholar
- Unseld M, Marienfeld JR, Brandt P, Brennicke A: The mitochondrial genome of Arabidopsis thaliana contains 57 genes in 366,924 nucleotides. Nature Genet. 1997, 15 (1): 57-61. 10.1038/ng0197-57.PubMedView ArticleGoogle Scholar
- Binder S, Marchfelder A, Brennicke A, Wissinger B: RNA editing in trans-splicing intron sequences of nad2 mRNAs in Oenothera mitochondria. J Biolog Chem. 1992, 267 (11): 7615-7623.Google Scholar
- Borner GV, Morl M, Wissinger B, Brennicke A, Schmelzer C: RNA editing of a group II intron in Oenothera as a prerequisite for splicing. Mol Gen Genet MGG. 1995, 246 (6): 739-744. 10.1007/BF00290721.View ArticleGoogle Scholar
- Kempken F, Hofken G, Pring DR: Analysis of silent RNA editing sites in atp6 transcripts of Sorghum bicolor. Curr Genet. 1995, 27 (6): 555-558. 10.1007/BF00314447.PubMedView ArticleGoogle Scholar
- Kubo N, Arimura S: Discovery of the rpl10 gene in diverse plant mitochondrial genomes and its probable replacement by the nuclear gene for chloroplast RPL10 in two lineages of angiosperms. DNA Res. 2010, 17 (1): 1-9. 10.1093/dnares/dsp024.PubMed CentralPubMedView ArticleGoogle Scholar
- Calixte S, Bonen L: Developmentally-specific transcripts from the ccmFN-rps1 locus in wheat mitochondria. Mol Genet Genom MGG. 2008, 280 (5): 419-426. 10.1007/s00438-008-0375-9.View ArticleGoogle Scholar
- Mundel C, Schuster W: Loss of RNA editing of rps1 sequences in Oenothera mitochondria. Curr Genet. 1996, 30 (5): 455-460. 10.1007/s002940050156.PubMedView ArticleGoogle Scholar
- Maier UG, Bozarth A, Funk HT, Zauner S, Rensing SA, Schmitz-Linneweber C, Borner T, Tillich M: Complex chloroplast RNA metabolism: just debugging the genetic programme?. BMC Biol. 2008, 6: 36-10.1186/1741-7007-6-36.PubMed CentralPubMedView ArticleGoogle Scholar
- Beaudet D, Nadimi M, Iffis B, Hijri M: Rapid mitochondrial genome evolution through invasion of mobile elements in Two closely related species of arbuscular mycorrhizal fungi. PloS one. 2013, 8 (4): e60768-10.1371/journal.pone.0060768.PubMed CentralPubMedView ArticleGoogle Scholar
- Mayfield SP, Yohn CB, Cohen A, Danon A: Regulation of chloroplast gene expression. Annu Rev Plant Physiol Plant Mol Biol. 1995, 46: 147-166. 10.1146/annurev.pp.46.060195.001051.View ArticleGoogle Scholar
- del Prete MJ, Vernal R, Dolznig H, Mullner EW, Garcia-Sanz JA: Isolation of polysome-bound mRNA from solid tissues amenable for RT-PCR and profiling experiments. Rna. 2007, 13 (3): 414-421. 10.1261/rna.79407.PubMed CentralPubMedView ArticleGoogle Scholar
- Sharpe RM, Dunn SN, Cahoon AB: A plastome primer set for comprehensive quantitative real time RT-PCR analysis of Zea mays: a starter primer set for other Poaceae species. Plant Methods. 2008, 4: 14-10.1186/1746-4811-4-14.PubMed CentralPubMedView ArticleGoogle Scholar
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