Table 1 Coding DNA Sequence (CDS) identifications of let- genes

lin-6/mcm-4

h92

C > *

Mini chromosome maintenance

RNAi

Confirmed¹

MCM4

Natural killer cell and glucocorticoid deficiency with DNA repair defect

[39]

let-354/dhc-1

h79

Q > *

Dynein heavy chain

Both

Confirmed¹

DYNC1H1

Charcot-Marie-Tooth disease, Mental retardation, Spinal muscular atrophy

[40–42]

let-502/rock

h392

Q > *

Rho associated kinase

RNAi

Confirmed¹

ROCK1

[43]

let-363/tor

h98

Splice variant

Tor kinase

Both

Confirmed¹

MTOR

pancreatic neuroendocrine tumors

[44, 45]

h420^a

Q > *

Confirmed³

h502^a

Splice variant

Confirmed³

let-603/air-2

h289

W > *

Aurora-related serine/threonine kinase

Both

Confirmed¹

AURKA

Susceptibility to colon cancer

[46]

let-512/vps-34

h797

P > S

phosphoinositide 3-kinase

Confirmed¹

PIK3C3

[47]

let-381/foxf

h107

splice variant

Forkhead transcription factor F

K.O.

Confirmed¹

FOXF2

[48]

let-607/bZip

h402

Q > *

Leucine zipper transcription factor

Both

Confirmed¹

CREB3L3

[49]

let-504/E01A2.4

h448

M > I

NFkB activating protein

Both

Confirmed¹

NKAP

let-152/ccb-1

h685

W > *

Calcium channel subunit

Confirmed²

CACNB2

Brugada syndrome 4

let-355/hel/T05E8.3

h81

Y > *

DEAD/H helicase

RNAi

Confirmed²

DHX33

let-362/rhel/Y71G12B.8

h86

R > *

DEAD/H RNA helicase

RNAi

Confirmed²

DDX27

let-366/aars-2

h112

Q > *

Alanine tRNA synthetase

RNAi

Confirmed²

AARS

Charcot-Marie-Tooth disease

[50]

let-368/inx-12

h121

W > *

Innexin gap junction

K.O.

Confirmed²

let-370/coq-1

h128

G > E

hexaprenyl pyrophosphate synthetase

K.O.

Confirmed²

PDSS1

Coenzyme Q10 deficiency, Parkinson’s disease

[51]

let-389/nars-1

h680

G > E

Asparagine tRNA synthetase

Both

Confirmed²

NARS

let-396/fars-1

h217

Q > *

Phenylalanine tRNA synthetase

RNAi

Confirmed²

FARSA

let-522/hlh-2

h735

W > *

Helix loop helix transcription factor

Both

Confirmed²

TCF3

Acute lymphoblastic leukemia

let-529/asd-2

h238

Q > *

KH domain containing RNA binding protein

RNAi

Confirmed²

QKI

Mental retardation

let-575/ptr-2

h345

W > *

Sterol sensing domain protein

RNAi

Confirmed²

PTCHD1

Autism spectrum disorders

[52–54]

let-585/inx-13

h784

W > *

Innexin gap junction

RNAi

Confirmed²

let-595/imb-1

h353

R > *

Importin

RNAi

Confirmed²

KPNB1

let-598/F27C1.6

h213

Q > *

U3 small nucleolar ribonucleoprotein

RNAi

Confirmed²

UTP14C

let-599/nath-10

h290

L > F

N-acetyl transferase

Both

Confirmed²

NAT10

let-608/ncbp-1

h706

Q > *

Nuclear cap binding protein

RNAi

Confirmed²

NCBP1

let-611/C48E7.2

h756

Q > *

RNA polymerase III subunit

RNAi

Confirmed²

POLR3C

let-612/apm-1

h466

splice variant

Adaptin subunit

RNAi

Confirmed²

AP1M1

let-365/sep-1

h108

W > *

Separase

Both

Confirmed²

ESPL1

Breast cancer oncogene

let-364/mat-1

h104

S > F

Anaphase promoting complex subunit

RNAi

Confirmed²

CDC27

let-101/npp-6

h242

W > *

Nuclear pore complex protein

Both

Confirmed²

NUP160

let-106/hcp-6

h787

C > Y

Condensin subunit

Both

Confirmed²

NCAPD3

let-379/tag-345

h127

W > *

Nucleolar protein complex member

RNAi

Confirmed²

WDR12

let-503/R12E2.2

h313

Q > *

Protein of unknown function

K.O.

Confirmed²

SUCO

let-517/spg-7

h264

G > E

Metalloprotease

Both

Confirmed²

AFG3L2

Spastic ataxia, Spinocerebellar ataxia

let-597/hcp-4

h349

E > *

Holocentromeric protein

RNAi

Confirmed²

CENPC

let-630/Y110A7A.19

h355^b

R > *

Pentatricopeptide repeat containing protein

RNAi

Confirmed²

PTCD3

h782^b

W > *

Confirmed²

let-646/pat-10

h233

G > E

Troponin C

RNAi

Confirmed²

TNNC1

Cardiomyopathy

let-526

h799^c

Q > *

SWI/SNF complex subunit

Both

Confirmed³

ARID1A

Mental retardation

h405^c

W > *

Confirmed³

let-129/zfh-2

h379

Q > *

zinc finger homeobox protein

Both

Prediction

ZFHX3, ZFHX4

Susceptibility to prostate cancer, Ptosis

let-147/rnp-6

h463

G > E

RNA splicing factor

RNAi range

Prediction

PUF60

Verheij syndrome

let-373/unc-73

h234

Del

Guanine nucleotide exchange factor

Both

Prediction

TRIO

let-377/lim-7

h110

W > *

LIM homeodomain protein

K.O.

Prediction

ISL2

let-378/dnj-21

h124

G > E

DnaJ domain containing protein

RNAi

Prediction

DNAJC15

let-380/knl-2

h80

W > *

Centromeric protein

Both

Prediction

let-382/nuo-2

h82

Q > *

Mitochondria complex I subunit

Both

Prediction

NDUFS3

Leigh syndrome, Mitochondrial complex I deficiency

let-383/T21G5.6

h115

W > *

Protein of unknown function

Prediction

let-384/C06A5.1

h84

Q > *

Integrator subunit

RNAi

Prediction

INTS1

let-385/teg-4

h85

splice variant

splicing factor

RNAi

Prediction

SF3B3

let-386/dbr-1

h117

G > E

RNA lariat-debranching enzyme

RNAi range

Prediction

DBR1

let-391/tag-146

h91

Q > *

Uncharacterized zinc finger protein

K.O.

Prediction

let-397/rpb-5

h228

Q > *

RNA polymerase II subunit

RNAi

Prediction

POLR2E

let-400/prpf-4

h269

D > G

Pre-mRNA processing factor

RNAi

Prediction

PRPF4B

[55]

let-509/unc-73

h142

W > *

Guanine nucleotide exchange factor

Both

Prediction

TRIO

let-527/nhr-23

h207

R > Q

Nuclear hormone receptor

Both

Prediction

RORC

let-534/ahcy-1

h260

Q > *

S-adenosylhomocysteine hydrolase

Both

Prediction

AHCY

Hypermethioninemia

let-581/unc-11

h725

A > V

clathrin adaptor protein

RNAi; Range

Prediction

PICALM

Acute lymphoblastic leukemia, Acute T-cell lymphoblastic leukemia

let-601/cuti-1

h281

Q > *

Cuticle regulatory protein

Both

Prediction

let-602/T09B4.9

h283

W > *

translocase

RNAi

Prediction

TIMM44

let-604/mdt-18

h293

splice variant

Mediator subunit

RNAi

Prediction

MED18

let-605/cye-1

h312

W > *

E-type cyclin

Both

Prediction

CCNE1

let-614

h138

Tested against F27C1.3 but did not confirm

let-376

h130

Tested against F55F8.3 but did not confirm

let-375

h241

Tested against imb-1 but did not confirm

let-387

h87

Tested against pnk-1 but did not confirm

let-515

h730

Tested against rpl-13 but did not confirm

let-501

h714

Tested against rpl-4 but did not confirm

let-361

h97

no candidate

let-531

h733

no candidate

let-576

h816

no candidate

let-518

h316

no candidate

let-523

h751

no candidate

let-525

h874

no candidate

let-584

h743

no candidate