Figure 5

Gene and isoform predictions. (Top) Novel alternative splicing of transcription elongation factor Supt5h differentially expressed in spermatogenesis. We see an ORF-preserving exon skipping event (A) that is frequently detected in samples after 12 dpp (B). (C) Predicted transcripts (variant 1 and variant 2 differ only by one retained intron). This prediction is based on our RNA-Seq data in combination with UCSC and Ensembl annotations and Genscan computational gene predictions. Only the protein coding part of the predicted transcripts is shown. The respective lengths of the predicted proteins are 566aa and 589aa. Both of these variants have a HMG box, which is a DNA-binding domain, towards their C-terminus, which is completely missed by the UCSC and Ensembl gene models. Expression at this locus peaks sharply at 12dpp. Note that there are a few differences in the boundaries of some exons between the predicted transcripts and the known annotation, which are invisible here.