Figure 5

Effect of constitutive activation of GNA1 or GNA3 and impact of phlp1 . Transcript ratios of phlp1 (A and B) and the G protein alpha subunit gene gna3 (C) in response to light upon growth on glycerol as carbon source are shown. qRT-PCR measurements show that both constitutively activated G alpha subunits (GNA1QL and GNA3QL) cause enhanced phlp1 transcript levels (A and B). PhLP1 positively influences gna1 transcript levels (Figure 4C), but not gna3 transcript levels (C). Strains were grown on Mandels-Andreotti minimal media with 1% (w/v) glycerol as carbon source for 24 hours in darkness (24DD) and then exposed to light for 15, 30, 60 or 120 minutes (minutes DL), respectively.