Figure 1
From: An integrated platform for bovine DNA methylome analysis suitable for small samples
Sequence of steps involved in the generation of methylome and transcriptome data from the same sample using EDMA platform. A quality control step prior to LM-PCR allows evaluation of the efficiency of cleavage by MSREs for methylome analysis. EDMA: EmbryoGENE DNA methylation analysis platform, ELMA: EmbryoGENE LIMS and Microarray Analysis, EMBV3: EmbryoGENE bovine transcriptomics microarray Version 3, LM-PCR: Ligation-mediated PCR, MSRE: Methyl-sensitive restriction endonuclease, RE: Restriction endonuclease (MseI).