Figure 4
From: An integrated platform for bovine DNA methylome analysis suitable for small samples
The feature of EDMA spiked-in controls and the quality control process of MSREs digestion assessment. (A) Location of sites cleaved by endonucleases within exogeous control DNA. (B) Difference in PCR threshold cycle (ΔΔCt) between un-cleaved (positive control) and cleaved samples. (C) Plot of residual number of copies and corresponding efficiency of cleavage by MSRE versus PCR cycle number, indicating that satisfactory cleavage (97%) is reached at the end of five PCR cycles.