Figure 5
From: An integrated platform for bovine DNA methylome analysis suitable for small samples
The two types of quality control (QC) plots generating after EDMA microarray hybridization and data analysis. (A) Signal intensity of control probes designed to overlap an MseI site. Probes corresponded to loci distributed throughout the bovine genome. The dashed horizontal line represents the limit of detection (mean intensity of negative controls plus four standard deviations). Intensities below this line indicate successful genomic fragmentation by MseI. (B) Cleavage of synthetic spiked-in control DNA pairs by MSRE, based on microarray signal. Signals from unprotected (unmethylated) fragments (right) are near background values.