Table 8 The sequences of the primers used in the study
From: The transcriptomic profile of peripheral blood nuclear cells in dogs with heart failure
Primer | Forward | Reverse | Annealing conditions |
|---|---|---|---|
ACTA2 | GTGGGGATGGGACAAAAGG | GAAAGCACCGCCTGAATAG | 60°C for 9 s |
MMP8 | CGATGCAGAAGAAACATGGA | TTGCTTGAAGGACCGTAGAT | 60°C for 10 s |
TIMP1 | CTTAAACCGGCGTTATGAGA | GGGGATGGATGAACAGGTAA | 60°C for 10 s |
GAPDH | CTGGGGCTCACTTGAAAGG | CAAACATGGGGGCATCAG | 59°C for 4 s |