Supplemental carnitine affects the microRNA expression profile in skeletal muscle of obese Zucker rats

BMC Genomics

Table 4 Validation of predicted target-mRNAs using qRT-PCR

Predicted target mRNAs	Related miRNA	Obese control	Obese carnitine
		Fold of control
	Up-regulated miRNAs
WNT5A	miR-10b-5p	1.00 ± 0.57	0.80 ± 0.46
UBE2A	miR-223-3p	1.00 ± 0.48	0.52 ± 0.10^*
HERC2	miR-208a-5p	1.00 ± 0.07	0.87 ± 0.08^*
ALCAM	miR-434-3p	1.00 ± 0.13	0.97 ± 0.16
ARPC5	miR-190a-5p	1.00 ± 0.18	0.73 ± 0.14^*
GNAI2	miR-30d-5p	1.00 ± 0.14	0.72 ± 0.07^*
FADS1	miR-347	1.00 ± 0.44	0.86 ± 0.11
PIAS1	miR-493-5p	1.00 ± 0.50	1.02 ± 0.59
CBLB	miR-29a-5p	1.00 ± 0.46	0.91 ± 0.25
SAMD4B	miR-451-5p	1.00 ± 0.38	0.98 ± 0.27
WSB1	miR-190b-5p	1.00 ± 0.41	0.67 ± 0.22
	Down-regulated miRNAs
SLC6A8	miR-466c-5p	1.00 ± 0.44	2.34 ± 0.52^*
ABCG1	miR-883-5p	1.00 ± 0.38	1.08 ± 0.26
IGF-1	miR-466b-1-3p	1.00 ± 0.28	2.26 ± 0.65^*
ITPR1	miR-21-3p	1.00 ± 0.29	1.37 ± 0.15^*
ACSL3	miR-3596c	1.00 ± 0.49	2.11 ± 0.87^*
SALL3	miR-3584-3p	1.00 ± 0.44	6.32 ± 2.47^*

Data are expressed as means ± SD and were presented as fold of the obese control group, which mean was set to 1; n = 6 rats/group. *Indicates significant difference to the obese control group (P < 0.05).

ISSN: 1471-2164