Table 5 Characteristics of specific primers used for validation of target-mRNAs by qRT-PCR
Gene | Forward Primer (3’-5’) | Reverse Primer (3’-5’) | Product length (bp) | Tm(°C) | NCBI GenBank |
|---|---|---|---|---|---|
Reference genes | |||||
ACTB | GACCTCTATGCCAACACAGT | CACCAATCCACACAGAGTAC | 154 | 60 | NM_031144.2 |
ATP5B | GCACCGTCAGAACTATTGCT | GAATTCAGGAGCCTCAGCAT | 203 | 60 | NM_134364.1 |
CANX | CCAGATGCAGATCTGAAGAC | CTGGGTCCTCAATTTCACGT | 175 | 60 | NM_172008.2 |
MDH1 | CAGACAAAGAAGAGGTTGCC | CGTCAGGCAGTTTGTATTGG | 206 | 60 | NM_033235.1 |
RPL13 | CTTAAATTGGCCACGCAGCT | CTTCTCAACGTCTTGCTCTG | 198 | 60 | NM_031101.1 |
TOP1 | GAAGAACGCTATCCAGAAGG | GCTTTGGGACTCAGCTTCAT | 137 | 60 | NM_022615.1 |
Target genes | |||||
ABCG1 | GCCATCCCTGTCTTGCTCTT | TCCTCTCGGTCCAAGCCATA | 143 | 56 | NM_053502.1 |
ACSL3 | GTAAAACTTGATTCCCGTTGAGA | GTGTCGCAGCCAGGATACA | 307 | 60 | NM_057107.1 |
ALCAM | TCGCTGACCCTCATCGTAGA | ATCGTCTGCCTCATCGTGTT | 321 | 60 | NM_031753.1 |
ARPC5 | GGGATGTCGAAGAACACGGT | GTAGGCATGAGTCCACCTCG | 142 | 60 | NM_001025717.1 |
CBLB | TTGAAGGGTGAAGATGCTTTTGAT | ACTGGAGCCTGGAGGTTTTG | 105 | 60 | NM_133601.1 |
FADS1 | CCACTACGCTGGTCAGGATG | AGCGCCTTATTCTTGGTGGG | 144 | 60 | NM_053445.2 |
GNAI2 | GCCGAGCGCTCTAAGATGAT | TGCTTGACGATGGTGCTCTT | 119 | 60 | NM_031035.3 |
HERC2 | CCTGACCACCGAGAGGAAAC | ACACCATCATCTGGATATCTGTT | 102 | 60 | NM_001107520.1 |
IGF-1 | CCCGGGACGTACCAAAATGAGCG | ATGTCAGTGTGGCGCTGGGC | 354 | 64 | NM_001082477.2 |
ITPR1 | ATGCCAGGAGGAAATGCGAA | CTCAGGGGTGGACTTGGTTC | 250 | 60 | NM_006236992.1 |
PIAS1 | ATGACCTGCTGGACGAACTG | ACTGTCGTGGACAATCGGAC | 355 | 60 | NM_001106829.2 |
SALL3 | CTCTTCTTGGTTTCCTAGGCGT | TCCGCCCACTTGAAGAACTC | 130 | 60 | NM_001108892.1 |
SAMD4B | TGTGGACCTCCCCTGCTTTG | GAGCAAAGGCACAGAAACCTG | 199 | 60 | NM_001107498.1 |
SLC6A8 | GGTCCCCTGTCATCGAGTTC | GAGGACCACGTAGGGGAATG | 197 | 60 | NM_017348.2 |
UBE2A | TGTGGAAACCACAGGACAACT | CAGTCACGCCAGCTTTGTTC | 327 | 60 | NM_001013933.1 |
WNT5A | TCCGCAGTCCTGCTTTGAAT | CAAAGCCACTCCTGGGCTTA | 156 | 60 | NM_022631.1 |
WSB1 | GAGTTCCCGGAATCAGACGG | CCGGAGCAAAAGCAACAGTC | 163 | 60 | NM_00104256.1 |