Figure 2

Heatmap of conserved miRNAs obtained by SOLiD sequencing during Senegalese sole early development. The complete heatmap (normalised to the number of trimmed reads, log2 transformed, Euclidean distance, complete linkage) has 320 rows (miRNAs) and 8 columns that correspond to 4 developmental stages (75Ep, 20S, hatching and metamorphosis) and two embryonic temperatures (15 and 21°C). For visualisation purposes, the heatmap was split in 3 panels: A) miRNAs with a low number of reads throughout development, B) miRNAs consistently expressed at high levels at all stages, and C) miRNAs with variable expression through development. Yellow and bue indicate higher and lower expression levels, respectively. miRNAs frequently referred to in the main text are highlighted in larger font.