Figure 3

Relative expressions of (A) miR-17a-5p, (B) miR-26a, (C) miR-130c, (D) miR-181a-5p, (E) miR-181a-3p, (F) miR-199a-5p and (G) miR-206-3p during Senegalese sole early development. Embryos were incubated at 15°C (blue bars) or 21°C (red bars) and transferred to 21°C after hatching. Six developmental stages were analysed (75% epiboly (75Ep), 20 somites (20S), hatching (hatch), pre-metamorphosis (pre-met), metamorphosis (met) and 30 days post-hatch (30 dph)) (N = 3 pools of embryos or larvae). Transcript levels were determined by qPCR and the control RNA Spike-in (Exiqon) was used to compare miRNA expression profiles. Significant differences between temperatures are indicated by asterisks (P < 0.05).