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Table 1 Functional annotation of the sequences from clusters I-VI

From: Transcriptome dynamics of Arabidopsis thaliana root penetration by the oomycete pathogen Phytophthora parasitica

Normalized hydridization signal

 

Blastx best hit

CL

Sequence

Myc

Zoo

App

2,5h

6h

10,5h

30h

FC

ID

Description

E value

I

CL1533Contig1

102

749

95

10813

1022

712

139

114

 

no hit

 

I

ppgam36a11r.1

127

131

336

1972

3482

13284

713

105

XP_002895059.1

elicitin-like protein INF4 [P. infestans]

3E-10

I

ppo3h07a24t.1

97

104

9105

1711

4473

1226

450

94

XP_002998010.1

secreted RxLR effector peptide protein, [P. infestans]

2E-13

I

CL1408Contig1

120

178

10915

1767

3707

5999

7470

91

XP_002896645.1

Amino Acid/Auxin Permease (AAAP) Family [P. infestans]

1E-134

I

ppo3h11p12t.1

296

334

4984

26369

10531

11172

2378

89

 

no hit

 

I

CL262Contig1

196

391

5444

14553

9894

3628

1081

74

 

no hit

 

I

CL380Contig1

108

120

7702

1192

3335

848

336

72

XP_002998010.1

secreted RxLR effector peptide protein, [P. infestans]

5E-19

I

CL1335Contig1

333

1011

13535

15140

18621

22234

10825

67

 

no hit

 

I

ppgam18a12r.1

112

116

119

6434

1077

814

167

57

 

no hit

 

I

ppo3h08i21t.1

122

128

6892

5236

3381

818

288

57

XP_002901148.1

carbohydrate-binding protein, [P. infestans]

2E-13

I

ppgam09e02r.1

100

110

116

5457

539

400

126

55

XP_001339148.2

PREDICTED: polymerase polyprotein-like [Danio rerio]

3E-53

I

CL42Contig1

132

134

7202

5692

3174

681

233

55

XP_002901145.1

hypothetical protein PITG_11596 [P. infestans]

1E-09

I

ppt4j31g10r.1

126

120

135

5163

639

381

163

43

 

no hit

 

I

ppo3h12o13t.1

118

154

4926

1188

4607

2779

1464

42

XP_002900991.1

conserved hypothetical protein [P. infestans]

2E-32

I

ppo3h06d21t.1

190

699

990

3799

7709

4416

2482

41

XP_002904652.1

ATP-binding Cassette (ABC) superfamily [P. infestans]

2E-17

I

ppo3h05p06t.1

96

96

2003

1513

3273

884

212

34

Q56TU4.1

S-adenosylmethionine synthase 1; Daucus carota

1E-13

I

CL382Contig1

180

190

5393

3807

3160

987

403

30

XP_002901148.1

carbohydrate-binding protein, [P. infestans]

6E-11

I

CL1026Contig1

125

144

900

1436

3606

2879

845

29

XP_002901054.1

secreted RxLR effector peptide protein, [P. infestans]

2E-15

I

CL366Contig1

122

228

274

3032

2717

1029

146

25

 

no hit

 

I

CL1109Contig1

135

138

3025

1229

3268

2982

2582

24

XP_002898673.1

ribonuclease, [P. infestans]

1E-89

II

CL1024Contig1

5212

1169

195

341

160

154

481

34

XP_500020.1

YALI0A12705p [Yarrowia lipolytica] emb

1E-13

II

ppgam23f03r.1

3620

1975

358

224

136

126

158

29

XP_002895853.1

sporangia induced phosphatidyl inositol kinase [P. infestans]

1E-142

II

CL1233Contig1

3966

6902

240

430

353

738

1651

29

XP_002899328.1

Ca2+−transporting ATPase endoplasmic reticulum type, [P. infestans]

0

II

CL1149Contig1

903

2978

308

207

297

436

462

14

XP_002905211.1

protein kinase, [P. infestans]

0

II

CD051670.1.1

1313

2611

2387

248

185

207

625

14

 

no hit

 

II

CL1407Contig1

10595

4542

757

1385

2211

3020

2338

14

XP_002908946.1

dihydroflavonol-4-reductase, [P. infestans]

1E-159

II

ppt4j11b07r.1

636

2408

175

259

352

341

343

14

XP_002903886.1

conserved hypothetical protein [P. infestans]

3E-99

II

CL772Contig1

4458

2341

605

336

991

969

1198

13

XP_002997937.1

conserved hypothetical protein [P. infestans]

0

II

CL864Contig1

9998

4700

787

1220

1070

1225

2643

13

XP_002898044.1

conserved hypothetical protein [P. infestans]

0

II

CL112Contig2

5791

4284

1116

2745

2166

1535

463

13

XP_002895909.1

ATP-binding Cassette (ABC) Superfamily [P. infestans]

0

II

ppgam04e11r.1

649

2816

231

262

328

349

557

12

 

no hit

 

II

CL1478Contig1

771

2708

235

250

347

341

514

12

XP_002907456.1

conserved hypothetical protein [P. infestans]

7E-76

II

ppgam07d02r.1

634

2263

196

293

225

275

345

12

XP_002998017.1

transmembrane protein, [P. infestans]

7E-71

II

CL471Contig1

5487

4101

582

848

477

756

1567

12

XP_002896830.1

conserved hypothetical protein [P. infestans]

1E-100

II

CL1485Contig1

1608

838

158

143

186

180

347

11

XP_002897750.1

short/branched chain specific acyl-CoA dehydrogenase, [P. infestans]

1E-125

II

CL349Contig1

1340

4691

549

502

543

431

729

11

XP_002909307.1

conserved hypothetical protein [P. infestans]

0

II

ppgam33a03r.1

2179

1553

202

508

579

938

701

11

XP_002905400.1

L-aminoadipate-semialdehyde dehydrogenase, [P. infestans]

1E-128

II

CL874Contig1

1039

2304

216

452

351

573

601

11

XP_002997338.1

HECT E3 ubiquitin ligase, [P. infestans]

7E-89

II

CL176Contig1

2239

5389

710

506

573

549

834

11

XP_002897342.1

conserved hypothetical protein [P. infestans]

8E-61

II

ppt4j38b11r.1

681

2149

332

246

230

202

432

11

XP_002896848.1

conserved hypothetical protein [P. infestans]

1E-108

III

ppo3h14g02t.1

142

11120

10795

18486

7479

2452

503

130

XP_002997786.1

glycoside hydrolase, [P. infestans]

1E-43

III

ppo3h14f01t.1

126

5446

7895

14463

5042

1485

437

115

 

no hit

 

III

CD051442.1.1

218

15744

14847

23012

17903

9763

5659

105

 

no hit

 

III

CL369Contig1

122

11151

4457

12379

4521

1277

268

102

XP_002997786.1

glycoside hydrolase, [P. infestans]

1E-138

III

CD051500.1.1

157

15890

5422

7601

5856

5131

1149

101

 

no hit

 

III

CL306Contig1

148

14591

3542

5108

3343

3089

710

99

XP_002898718.1

conserved hypothetical protein [P. infestans]

1E-146

III

CL804Contig1

130

9313

6270

2748

2823

2240

1429

72

XP_002904112.1

sulfatase-like protein [P. infestans]

1E-132

III

CL515Contig1

131

7782

2639

1063

1153

1103

519

59

XP_002904421.1

conserved hypothetical protein [P. infestans]

0

III

CD051686.1.1

135

6845

2453

1660

868

844

290

51

 

no hit

 

III

CL1187Contig1

181

9106

1049

3912

5197

6878

5043

50

XP_002896478.1

poly [ADP-ribose] polymerase, [P. infestans]

1E-174

III

CF891673.1.1

157

5465

5871

2105

4405

2699

1995

37

 

no hit

 

III

CL232Contig1

200

7317

3970

3044

4343

2281

3612

37

XP_002904949.1

conserved hypothetical protein [P. infestans]

1E-168

III

CL1195Contig1

135

4941

2491

3037

2412

1683

724

37

XP_002895053.1

conserved hypothetical protein [P. infestans]

1E-129

III

CL181Contig1

276

7495

9615

1909

1423

1212

1047

35

XP_002900508.1

conserved hypothetical protein [P. infestans]

1E-119

III

ppo3h09b05t.1

172

5371

703

1742

819

937

463

31

ABG80552.1

cell 5A endo-1,4-betaglucanase [P. ramorum]

2E-97

III

ppo3h07h24t.1

112

3238

1398

543

840

680

469

29

XP_002906516.1

transmembrane protein, [P. infestans]

1E-117

III

ppo3h13d10t.1

115

3128

1867

885

647

518

171

27

XP_002998505.1

GPI-anchored serine-rich elicitin INL3b-like protein [P. infestans]

1E-38

III

CL449Contig1

125

3223

2186

1589

1510

843

410

26

XP_002898095.1

conserved hypothetical protein [P. infestans]

7E-96

III

ppt4j29b11r.1

158

3765

2184

1048

557

849

399

24

XP_002902649.1

Drug/Metabolite Transporter (DMT) Superfamily [P. infestans]

1E-108

III

CL330Contig1

1867

23600

43890

40265

40618

35214

14295

24

XP_002904340.1

conserved hypothetical protein [P. infestans]

1E-102

IV

CL8Contig1

44525

163

325

452

825

9662

7144

274

XP_002899844.1

conserved hypothetical protein [P. infestans]

1E-152

IV

CL639Contig1

22961

606

806

221

192

166

413

139

ABG23233.1

unknown [Hyaloperonospora parasitica]

1E-36

IV

ppo3h02e04t.1

19582

157

3139

1151

2639

7487

7743

124

XP_002900352.1

Proton-dependent Oligopeptide Transporter (POT) Family [P. infestans]

8E-98

IV

ppgam01h01r.1

17201

173

339

204

164

142

881

121

XP_002901432.1

conserved hypothetical protein [P. infestans]

6E-33

IV

ppo3h10m18t.1

25224

224

6090

1667

2610

4991

19398

113

 

no hit

 

IV

CL90Contig1

13934

167

1552

489

7712

8560

18029

108

XP_002999240.1

pyrophosphate vacuolar membrane proton pump, [P. infestans]

0

IV

CL32Contig1

14467

189

275

148

258

429

4440

97

AAM18483.1

AF494014_1 exo-1,3-beta-glucanase [P. infestans]

0

IV

ppgam24d04r.1

13873

149

200

667

295

697

973

93

XP_002907089.1

protein kinase, [P. infestans]

1E-127

IV

CL210Contig1

12224

133

178

647

362

905

11665

92

XP_002909387.1

conserved hypothetical protein [P. infestans]

7E-69

IV

ppgam36c10r.1

16676

187

4612

1365

566

1463

8770

89

XP_002904661.1

Major Facilitator Superfamily (MFS) [P. infestans]

1E-126

IV

CL119Contig1

10489

126

163

144

246

461

494

84

XP_002900778.1

Annexin (Annexin) Family [P. infestans]

1E-166

IV

ppgam37e05r.1

15664

342

205

390

190

188

2589

83

XP_002907653.1

oxidoreductase, [P. infestans]

4E-85

IV

CL71Contig1

10898

137

1568

339

1504

4481

9186

79

ABH11757.1

elicitin-like protein 6 precursor [P. nicotianae]

4E-44

IV

ppo3h07a23t.1

9263

118

124

161

162

175

1752

78

XP_002907297.1

P-type ATPase (P-ATPase) Superfamily [P. infestans]

1E-120

IV

CL92Contig1

12589

213

871

357

5799

12282

16142

76

XP_002998388.1

carbohydrate-binding protein, [P. infestans]

1E-127

IV

ppgam07a10r.1

8342

249

671

275

1663

9237

18817

76

XP_002903354.1

mucin-like protein [P. infestans]

8E-38

IV

ppgam02h08r.1

4632

136

625

454

3462

10250

8890

75

XP_002896569.1

glucosylceramidase, [P. infestans]

1E-109

IV

ppt4j09a01r.1

8222

115

199

259

220

399

8676

75

XP_002909387.1

conserved hypothetical protein [P. infestans]

2E-46

IV

ppgam18e03r.1

15537

715

353

538

227

207

2704

75

XP_002902980.1

acyl-CoA synthetase short-chain family member, [P. infestans]

1E-124

IV

CL758Contig1

12392

169

311

676

908

2489

3671

73

XP_002904197.1

glucosylceramidase, [P. infestans]

1E-156

V

ppo3h06f02t.1

409

777

214

8582

1544

1290

509

40

XP_002997773.1

conserved hypothetical protein [P. infestans]

7E-78

V

ppgam12h06r.1

781

868

171

5385

359

274

138

39

 

no hit

 

V

ppgam31f01r.1

119

197

159

2339

520

353

164

20

 

no hit

 

V

ppt4j25b05r.1

97

176

126

1823

424

369

127

19

AAR21576.1

heat shock protein 70 [P. nicotianae]

2E-20

V

CL1505Contig1

480

596

349

5757

3617

1585

1274

17

XP_002895485.1

ATP-binding Cassette (ABC) superfamily [P. infestans]

1E-136

V

ppt4j21a06r.1

445

118

199

1866

303

357

311

16

XP_002895988.1

maltose O-acetyltransferase, [P. infestans]

5E-86

V

ppgam36f04r.1

108

116

171

1707

275

240

140

16

 

no hit

 

V

ppgam17d07r.1

159

458

362

2083

608

514

333

13

XP_002906389.1

conserved hypothetical protein [P. infestans]

2E-65

V

CL344Contig1

138

179

483

1684

534

446

257

12

 

no hit

 

V

ppgam04f05r.1

128

167

462

1554

521

297

166

12

 

no hit

 

V

ppo3h02b24t.1

112

407

356

1344

495

796

671

12

XP_002895175.1

conserved hypothetical protein [P. infestans]

5E-51

V

ppo3h06n03t.1

129

135

526

1544

284

204

175

12

NP_001063268.1

Os09g0438100 [Oryza sativa Japonica Group] dbj

0,000009

V

ppt4j01f03r.1

106

122

165

1257

236

187

145

12

XP_002903318.1

conserved hypothetical protein [P. infestans]

1E-15

V

ppo3h03i13t.1

161

608

253

1692

729

454

216

11

XP_002904470.1

conserved hypothetical protein [P. infestans]

5E-97

V

ppo3h01k13t.1

126

418

348

1275

506

395

230

10

XP_002903425.1

conserved hypothetical protein [P. infestans]

3E-45

V

ppt4j36c04r.1

291

127

168

1285

233

258

241

10

XP_002182839.1

predicted protein [Phaeodactylum tricornutum CCAP 1055/1]

4E-51

V

ppo3h12p16t.1

564

1450

1037

5589

1138

1127

581

10

XP_002898514.1

conserved hypothetical protein [P. infestans]

1E-36

V

ppo3h03o24t.1

240

119

230

1158

360

368

185

10

 

no hit

 

V

ppt4j38e02r.1

102

102

100

943

200

196

117

9

 

no hit

 

V

ppgam22f11r.1

122

120

153

1082

249

223

145

9

XP_002896344.1

conserved hypothetical protein [P. infestans]

2E-14

VI

ppo3h12n08t.1

104

106

37783

103

124

120

116

365

 

no hit

 

VI

ppo3h10g01t.1

113

131

9703

121

207

111

112

87

 

no hit

 

VI

ppo3h12n20t.1

104

128

8715

707

225

478

128

84

XP_002909505.1

mannitol dehydrogenase, [P. infestans]

5E-41

VI

ppo3h13j21t.1

92

94

4826

93

122

112

96

52

 

no hit

 

VI

CL613Contig1

117

129

5082

224

1021

1359

1803

43

XP_002904550.1

folate-Biopterin Transporter (FBT) family [P. infestans]

0

VI

ppo3h03f02t.1

132

996

5251

350

169

144

142

40

XP_002904736.1

conserved hypothetical protein [P. infestans]

2E-69

VI

ppo3h05l14t.1

95

173

3749

261

179

118

114

39

XP_002905279.1

cleavage induced hypothetical protein [P. infestans]

5E-86

VI

CL419Contig1

139

323

5306

737

1879

924

595

38

ABB22029.1

cell 12A endoglucanase [P. sojae]

1E-117

VI

ppo3h06d03t.1

112

124

4159

443

816

580

173

37

XP_002904437.1

conserved hypothetical protein [P. infestans]

1E-112

VI

ppt4j22c09r.1

118

593

3355

2610

422

405

321

28

XP_002909523.1

Amino Acid-Polyamine-Organocation (APC) Family [P. infestans]

1E-115

VI

ppo3h04p13t.1

113

118

2658

565

195

151

129

24

XP_002901038.1

carbohydrate-binding protein, [P. infestans]

1E-58

VI

CL118Contig1

105

106

2438

176

140

160

118

23

XP_002902444.1

conserved hypothetical protein [P. infestans]

1E-107

VI

CL999Contig1

117

131

2593

402

881

527

270

22

XP_002901373.1

conserved hypothetical protein [P. infestans]

1E-86

VI

CL1422Contig1

138

172

3038

797

222

254

913

22

XP_002904844.1

ATP-binding Cassette (ABC) Superfamily [P. infestans]

1E-165

VI

CL317Contig1

160

124

2693

276

585

516

953

22

XP_002901401.1

conserved hypothetical protein [P. infestans]

7E-26

VI

CL1406Contig1

105

112

1979

156

169

164

117

19

ABG23232.1

N-acetyltransferase-like protein [Hyaloperonospora parasitica]

3E-49

VI

CL448Contig1

237

228

4124

1663

1472

745

545

18

XP_002900313.1

cutinase, [P. infestans]

1E-103

VI

CL1513Contig1

124

124

2132

264

650

571

264

17

XP_002903927.1

pectin lyase, [P. infestans]

2E-61

VI

CL1176Contig1

106

116

1784

152

122

124

108

17

XP_002999045.1

conserved hypothetical protein [P. infestans]

1E-142

VI

ppo3h13a15t.1

97

100

1585

101

119

110

102

16

XP_002518625.1

conserved hypothetical protein [Ricinus communis]

1E-51

  1. Annotation proposed for the 20 genes with the highest fold change in each cluster (CL) is presented. For all sequences, normalized hybridization signal in each condition (mean of the two biological replicates) is indicated. Maximum fold change (max FC) observed between the different biological samples and BlastX best hit result (E value < 1E-05) against NCBI non-redundant (NR) protein database are presented.