Figure 2

Comparative fermentations with parent yeast BY4743 and nine stuck mutants in CDGJM_200. Fermentations (100 mL) were performed in triplicate in CDGJM_200 at 28°C whereby the deletants were compared directly with the parental strain BY4743. Growth was monitored as optical density at 600 nm (open symbols). Sugar consumption was monitored enzymatically and reported as total or individual sugars (solid symbols). The data are arranged in three rows (A-C) of three plots each (left to right). The first plot in each row depicts total residual sugar. Glucose-only and fructose-only information is shown to the right of the corresponding total sugar plot. The nine deletion strains (deletants) which result in arrested or ‘stuck’ fermentation are shown in rows A (Δnpt1, Δplc1, Δssq1), B (Δdoa4, Δsin3, Δzap1) and C (Δptk2, Δtps1, Δtps2).