Figure 4

Clustering analysis of variant genes during myogenesis. Expression profiles of the 67 genes whose transcript levels changed significantly during myogenesis of mouse satellite cells were used. The resulting tree of hierarchical clustering (see Methods) was split into eight groups (1 to 8). Group 8 included 28 genes and was split again into six smaller groups (1 to 6) to obtain more homogenous groups. A. Number of genes in clusters and number of genes found in group A and group B as defined in Table 1. Mean expression profiles for C2C12 (pink) and MSC (red) of clusters 3, 5, 7 8(4) and 8(6) are shown in C, D, E, F and G. B. Expression map of the five major clusters (boxed in red): 3, 5, 7, 8(4) and 8(6). C-G. Mean expression profiles obtained for C2C12 (pink) and MSC (red): y-axis represents the standard log2 of expression levels (RQ for relative quantity). Error bars indicate standard deviation of average expression.