All-Food-Seq (AFS): a quantifiable screen for species in biological samples by deep DNA sequencing

BMC Genomics

Table 1 Mapping results from simulated datasets

Species	Reads assigned	Proportion [%]	Target value [%]	Difference abs. [%]	Difference rel. [%]
Cattle	26,555	2.95	3	0.05	1.67
Horse	224,077	24.91	25	0.09	0.36
Human	8,969	1.00	1	0.00	0.00
Pig	89,421	9.94	10	0.06	0.60
Rat	9,042	1.01	1	0.01	1.00
Sheep	541,432	60.19	60	0.19	0.32

Simulated quantification of sequence reads obtained from six different genomes using the AFS pipeline. “Difference abs.” shows the difference between the proportions of reads, as determined by AFS (“proportion”), relative to the expected amounts existing in the sample (“target value”). “Difference rel.” is calculated by dividing “Difference abs.” by the expected proportion value.

ISSN: 1471-2164