Figure 3

Ectopic miR-K12-11 and miR-155 expression. A and B. BJAB (A) and TIVE (B) cells stably express GFP after foamy virus transduction and purification by Fluorescence Activated Cell Sorter. C. Expression and copy number analysis of miR-K12-11 in transduced cells compared to the PEL cell line BCBL- using stem-loop qRT-PCR. The absolute numbers of miR-K12-11 from transduced cells was lower than in BCBL-1 indicating that ectopic expression was not to super-physiological levels. D and E. Expression levels of miR-155 in BJAB cells. There was endogenous expression of miR-155, although the ectopic miRNA expression was higher. Multiplicity of infection (MOI, i.e. copies per cell) did not result in consistent and significant changes in the miRNA expression levels, and was therefore not separately considered in further analysis. Y axis: relative quantity to the reference RNU66.