Table 3 Primer sequences for quantitative real-time rtPCR of selected potential biomarker genes
Target gene | Genbank accession | Forward (5’-3’) | Reverse (5’-3’) | Product size (bp) | Efficiency (%) | Annealing temp. (°C) |
|---|---|---|---|---|---|---|
AIFM1 | JT833124 | CCCTACTGGCATCAGTCCAT | TGTCCTTCTGCTCGGGTACT | 250 | 96.8 | 59.1 |
HSP90AB1 | NM_001123532 | TCATGGACAGCTGTGAGGAG | CCAGCTTGAGGTTCTTGGAG | 234 | 95.5 | 56 |
SDHD | BT071922 | GCGCATGCACTTAGTCAAAA | GGTGCATTTTTCTTCCCAAA | 248 | 90.8 | 59.1 |
P4HB | BT072340 | TAAGCGTGATTGCGTGAGTC | TGTGATGGAATGCGTTTGTT | 175 | 95.7 | 56 |
COX6B1 | BT125515 | GACAATGCTTGGCACATACG | TGTCAGCAGATGCAGAGTCC | 218 | 95.4 | 59.1 |
PRDX3 | BT046676 | CTAAGTGGGCTCCAGCTGTC | ATGATCTCTGTCGGGCAAAC | 163 | 96.4 | 56 |
P53 | EZ772237 | GAGGAGATCAACCTGAAGAAGCA | AGGCCTCCTTCATAGCACGTT | 91 | 100 | 63.4 |
JAK1 | BT057852.1 | ACTAACTGGCATGGGACCAG | CCAGACCCTTCTGGAAATCA | 172 | 95.2 | 59.1 |
18S | AJ427629 | TGTGCCGCTAGAGGTGAAATT | GCAAATGCTTTCGCTTTCG | 61 | 95.4 | 59.1 |
RPL1 | CB516726 | ACTATGGCTGTCGAGAAGGTGCT | TGTACTCGAACAGTCGTGGGTCA | 118 | 95.3 | 60 |