Figure 9

Csi-miR164 targeted Cs5g10870 was verified in vivo. (a) Expression levels of mi164 and Cs5g10870 were detected during the fruit development by qRT-PCR. U4 and ACTIN were used as loading controls in qRT-PCR Data from the qRT-PCR experiments are represented as the mean plus SD of n = 3 biological replicates. (b) Four overexpression vectors were constructed for transient expression system in tobacco. Vector ox-miR164 overexpress csi-miR164, ox-miRCon overexpress a control miRNA, ox-10TS + GFP overexpress a GFP gene carrying target site of miR164 in Cs5g10870, ox-10MTS + GFP overexpress a GFP gene carrying a modified target site. (c) Vectors used in tobacco co-expression assays in each lane were shown in the table. (d) Co-infiltrated leaves and control leaves were photographed at the 3rd day after infiltration under bright light. (e) Same leaves were photographed at the 3rd day after infiltration under UV light (wavelength = 365 nm).