Table 1 Genes included in PCR array

Jun

Fos and Jun dimerize to form AP-1, involved in cell proliferation, differentiation, and transformation

Fos

PCNA, proliferating cell nuclear antigen

DNA synthesis, cell-cycle control, and DNA-damage response and repair

CCND1, cyclin D1

Regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition

TERT1, telomerase reverse transcriptase

Maintains telomere ends, chromosomal repair

mycN

Transcription factor amplified or overexpressed in variety of tumors

cMYC

Transcription factor activated upon various mitogenic signals such as Wnt, Shh and EGF

gli3

Transcription factor mediating sonic hedgehog signaling

SOX2

Transcription factor with roles in embryonic development, cell fate determination, stem cell maintenance

Oct4

Involved in the self-renewal of undifferentiated embryonic stem cells.

Klf4

Indicator of stem-like capacity in embryonic stem cells

VEGF1

Endothelial cell mitogen

ANGPT1, angiopoietin 1

Involved in vascular development and angiogenesis

CAV1, caveolin

Scaffolding protein, possible tumor suppressor

Wnt5A

Secreted signaling protein, activates beta catenin transcriptional activity

Wnt11

Secreted signaling protein, implicated in oncogenesis and in several developmental processes

SNAI2, snail2

Transcriptional repressor involved in epithelial-mesenchymal transitions and has antiapoptotic activity.

TGFbeta

Member of a family of peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types

IGF1, insulin like growth factor

Growth and anabolic effects

IL2

Cytokine regulates lymphocyte activity

IL10

Anti-inflammatory cytokine

PTGS1, COX1, prostaglandin G/H synthase and cyclooxygenase)

Converts arachidonic acid to prostaglandin