Figure 2

Novel miRNAs and clusters detected in E. granulosus. (a) The location of miRNA gene clusters in E. granulosus scaffolds. The rightward arrow indicates the location of the miRNA gene at the positive strand, while the leftward arrow indicates the miRNAs at the minus strand. The secondary structures of Echinococcus cluster containing new-17, new-24, new-61 and new-7 (b) and the cluster containing new-12 and new-22 (c) were predicted by mfold. The sequence of the mature miRNAs is shaded in grep. Limited by the size of the miRNA cluster, we used “N” instead of 5464 nt RNA sequences at the location between new-61 and new-7.