Figure 1
Validation of β-catenin siRNA. DLD1 and SW480 cells were treated with β-catenin siRNA (siβcat) or β-galactosidase siRNA (siβgal) and the protein expression levels of β-catenin were determined by immuno detection using an anti-β-catenin antibody. Actin was used as a loading control (A). The activity of the β-catenin dependent reporter gene 8×TOPflash was determined in DLD1 and SW480 cells after treatment of these cells with β-catenin siRNA or β-galactosidase siRNA. The experiments were performed in duplicates with error bars representing standard deviation (B).