Table 1 Statistics of DGE sequencing at the three developmental stages

Raw tag

Aml

6,000,000

6,000,000

6,000,000

6,000,000

9,020,722

9,018,194

42,038,916

Acc

6,000,000

6,000,000

6,000,000

6,000,000

6,000,000

6,000,000

36,000,000

Distinct tag

Aml

211,272

198,230

157,958

160,974

289,909

256,732

1,275,075

Acc

215,863

219,554

144,442

130,883

170,217

213,917

1,094,876

Clean tag

Aml

5,882,059(98.03%)

5,890,202(98.17%)

5,910,901(98.52%)

5,911,105(98.52%)

5,423,816(60.13%)

5,849,152(64.86%)

34867235(82.94%)

Acc

5,883,978(98.07%)

5,878,500(97.98%)

5,917,830(98.63%)

5,923,277(98.72%)

5,904,132(98.40%)

5,871,384(97.86%)

35,379,101(98.28%)

Distinct clean tag

Aml

100,676(47.65%)

95,104(47.98%)

77,435(49.02%)

80,286(49.88%)

61,548(21.23%)

52,987(20.64%)

468,036(36.71%)

Acc

106,580(49.37%)

104,133(47.43%)

71,482(49.49%)

63,646(48.63%)

82,699(48.58%)

95,883(44.82%)

524,423(47.90%)

All tag mapping to gene

Aml

3,958,194(67.29%)

4,039,673(68.58%)

4,749,009(80.34%)

4,687,158(79.29%)

4,578,511(84.41%)

5,206,584(89.01%)

27,219,129(78.07%)

Acc

2,476,714(42.09%)

2,570,588(43.73%)

2,740,538(46.31%)

2,755,741(46.52%)

2,422,568(41.03%)

3,091,826(52.66%)

16,057,975(45.39%)

Unambiguous tag mapping to gene

Aml

3,549,912(60.35%)

3,579,074(60.76%)

3,550,900(60.07%)

3,551,385(60.08%)

4,465,416(82.33%)

5,157,312(88.17%)

23,853,999(68.41%)

Acc

2,370,309(40.28%)

2,512,797(42.75%)

2,520,841(42.60%)

2,321,965(39.20%)

2,404,037(40.72%)

3,014,975(51.35%)

15,144,924(42.81%)

Mapping to genome

Aml

1,100,732(18.71%)

1,025,724(17.41%)

732,560(12.39%)

751,100(12.71%)

558,499(10.30%)

458,366(7.84%)

4,626,981(13.27%)

Acc

925,681(15.73%)

1,002,791(17.06%)

659,768(11.15%)

492,653(8.32%)

923,103(15.63%)

773,385(13.17%)

4,777,381(13.50%)

All tag-mapped genes

Aml

8,060(72.88%)

7,705(69.67%)

7,725(69.85%)

7,464(67.49%)

7,388(66.8%)

6,761(61.13%)

45,103(67.97%)

Acc

7,317(66.16%)

7,385(66.77%)

6,656(60.18%)

6,450(58.32%)

6,961(62.94%)

7,045(63.7%)

41,814(63.01%)

Unambiguous tag-mapped genes

Aml

7,826(70.76%)

7,491(67.73%)

7,503(67.84%)

7,255(65.6%)

7,184(64.95%)

6,578(59.48%)

43,837(66.06%)

Acc

7,077(63.99%)

7,157(64.71%)

6,469(58.49%)

6,250(56.51%)

6,743(60.97%)

6,824(61.7%)

40,520(61.06%)

Unknown tag

Aml

823,133(13.99%)

824,805(14.00%)

429,332(7.26%)

472,847(8.00%)

286,806(5.29%)

184,202(3.15%)

3,021,125(8.66%)

Acc

2,481,583(42.18%)

2,305,121(39.21%)

2,517,524(42.54%)

2,674,883(45.16%)

2,558,461(43.33%)

2,006,173(34.17%)

14,543,745(41.11%)