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Table 3 Comparison of the expression profiles of 26 genes by RNA-seq and quantitative real-time RT-PCR

From: Deep sequencing–based comparative transcriptional profiles of Cymbidium hybridum roots in response to mycorrhizal and non-mycorrhizal beneficial fungi

Gene ID

Annotation

M/CK (log2fold)

Z/CK (log2fold)

MZ/CK (log2fold)

  

RNA-seq

qRT-PCR

RNA-seq

qRT-PCR

RNA-seq

qRT-PCR

Unigene 1724_All

ethylene receptor homolog2

1.72

1.59 ± 0.24

1.19

0.73 ± 0.09

1.87

1.51 ± 0.05

Unigene 1869_All

LysM domain receptor-like kinase 3-like

4.26

4.29 ± 0.06

0.51

0.98 ± 0.19

3.97

3.85 ± 0.03

Unigene 2625_All

calmodulin binding protein, putative

1.19

0.95 ± 0.16

0.87

0.38 ± 0.15

1.30

0.64 ± 0.10

Unigene 4130_All

sugar transporter ERD6-like 7-like

1.74

1.70 ± 0.21

0.84

1.01 ± 0.05

2.03

1.63 ± 0.38

Unigene 4239_All

boron transporter 2

0.96

0.58 ± 0.25

-2.17

-3.07 ± 0.06

0.61

0.27 ± 0.39

Unigene 6086_All

inorganic phosphate transporter, putative

3.01

2.81 ± 0.34

2.49

2.90 ± 0.26

3.40

3.06 ± 0.10

Unigene 8895_All

Xyloglucan endotransglucosylase/hydrolase

2.03

0.58 ± 0.66

2.55

1.74 ± 0.49

1.89

-0.07 ± 0.38

Unigene 10786_All

equilibrative nucleoside transporter

3.95

4.71 ± 0.11

-0.21

0.42 ± 0.08

3.94

4.41 ± 0.05

Unigene 12197_All

ethylene-responsive element binding factor 4

2.33

1.83 ± 0.53

3.12

2.72 ± 0.39

3.37

2.74 ± 0.11

Unigene 13078_All

glutathione peroxidase

4.20

2.62 ± 0.15

1.67

1.04 ± 0.27

3.95

2.45 ± 0.06

Unigene 13219_All

transmembrane copper transporter

-6.93

-1.01 ± 0.03

8.05

8.15 ± 0.44

6.51

4.65 ± 0.06

Unigene 13639_All

ATPase 7, plasma membrane-type-like

4.24

4.39 ± 0.09

0.52

0.32 ± 0.11

3.94

3.81 ± 0.09

Unigene 14415_All

major facilitator family protein

-2.13

-1.55 ± 0.50

-0.97

-1.13 ± 0.86

-2.36

-1.91 ± 0.50

Unigene 15955_All

sorbitol transporter

2.32

2.86 ± 0.38

0.87

1.54 ± 0.21

2.27

2.20 ± 0.07

Unigene 13573_All

cellulose synthase A catalytic subunit 4 [UDP-forming]-like

4.76

7.26 ± 0.91

4.94

7.61 ± 0.87

4.67

6.50 ± 0.89

Unigene 2569_All

transcription factor, putative

2.18

2.68 ± 0.30

0.00

0.38 ± 0.20

2.00

2.41 ± 0.32

Unigene 14999_All

NAC domain-containing protein 43-like

4.19

3.73 ± 0.36

4.60

4.53 ± 0.32

4.32

3.09 ± 0.18

Unigene 16297_All

CBL-interacting serine/threonine-protein kinase 11

1.80

1.34 ± 0.35

1.61

1.80 ± 0.18

2.17

1.55 ± 0.35

Unigene 10727_All

zinc finger CCCH domain-containing protein 66-like

2.22

2.07 ± 0.24

3.09

2.82 ± 0.04

3.53

2.87 ± 0.01

Unigene 15958_All

chitinase 4-like

20.38

13.75 ± 0.15

0.00

-0.04 ± 0.34

20.09

13.40 ± 0.21

Unigene 4429_All

laccase-4-like

2.35

2.35 ± 0.29

2.81

3.38 ± 0.06

1.96

1.81 ± 0.19

Unigene 11039_All

auxin efflux facilitator PIN1

3.42

3.27 ± 0.39

3.74

2.84 ± 0.07

3.77

3.00 ± 0.25

Unigene 15779_All

Xylem serine proteinase 1 precursor, putative

1.54

2.09 ± 0.30

2.40

3.21 ± 0.13

1.79

2.32 ± 0.20

Unigene 2376_All

peptide transporter PTR3-A-like

2.92

2.38 ± 0.37

-1.01

-1.44 ± 0.45

2.79

1.70 ± 0.14

Unigene 2386_All

oligopeptide transporter 1-like

4.51

4.90 ± 0.49

1.66

2.51 ± 0.46

4.57

4.86 ± 0.49

Unigene 13170_All

gibberellin 20-oxidase

1.66

2.13 ± 0.05

0.03

0.45 ± 0.16

1.14

1.00 ± 0.27

  1. Fold changes of selected C. hybridum trasncripts (±standard deviation) were logarithmic transformed which were determined by RNA-seq and qRT-PCR in root tissues treated with isolates ML01 (M) , ZH3A-3 (Z) and ML01+ ZH3A-3 (MZ) at 15 dpi as compared to mock-inoculated control (CK).