Table 2 Polymerase chain reaction (PCR) and sequencing primers used to amplify portions of the mtDNA and Y chromosome for phylogenetic analysis to verify Mauritius origin of the resequenced cynomolgus macaques from the Goswami et al.[28] study
From: Genetic substructure in cynomolgus macaques (Macaca fascicularis) on the island of Mauritius
Gene | Primer Name | Sequence (5′ to 3′) |
|---|---|---|
mtDNA | Â | Â |
HSVI; partial cytb | Â | Â |
| Â | Forward | CCG CCC ACT CAG CCA ATT CCT GTT CT |
| Â | Reverse | CCC GTG ATC CAT CGA GAT GTC TT |
Y chromosome | Â | Â |
SRY | Â | Â |
| Â | SW2 | CTT GAG AAT GAA TAC ATT GTC AGG G |
| Â | SW3B | AGG TCT TTG TAG CCA ATG TTA CCC G |
| Â | F1 | AGT GAA GCG ACC CAT GAA YG |
| Â | R1 | GTA TCC CAG MTG CTT GCT GAT C |
TSPY | Â | Â |
| Â | TSPY-A | AGC CAG GAA GGC CTT TTC TCG |
| Â | TSPY5R | CTG TGC ATA AGA CCA TGC TGA G |
| Â | 470Â F | CGG CAG TTC TCT GCA T |
| Â | E690R | TCG RCA TGG ATA AGA CGG AC |