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Figure 4 | BMC Genomics

Figure 4

From: Small non-coding RNA profiling and the role of piRNA pathway genes in the protection of chicken primordial germ cells

Figure 4

qPCR and knockdown analysis of CIWI and CILI . (A) qPCR quantitative expression analysis of the chicken piRNA pathway genes CIWI and CILI in PGCs, stage X blastoderms, GSCs, and CEFs. (B) Knockdown of CIWI and CILI in chicken PGCs. Three siRNAs each for the knockdown of CIWI and CILI were transfected into PGCs using lipofection. Approximately 48 h after transfection, knockdown efficiency was examined by qPCR amplification of CIWI and CILI. The expression of CIWI and CILI was normalized against that of GAPDH. Bars indicate the SEM of triplicate analyses. **P < 0.01 vs. control and ***P < 0.001 vs. control.

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BMC Genomics

ISSN: 1471-2164

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