Table 1 Coverage of BV-2Â N-glycoproteome by various approaches
Experimental scheme 3 | FDR < 1% &18O deamidation | N!P-[S/T/rarely C] motif | |||||
|---|---|---|---|---|---|---|---|
Approach | Replicates | Redundant N-glycosites | Unique N-glycosites | Unique N-glycoproteins | Redundant N-glycosites | Unique N-glycosites | Unique N-glycoproteins |
WCC | Biological replicate 1 | 1250 | 545 | 297 | 1052 | 396 | 231 |
Biological replicate 2 | 1501 | 576 | 305 | 1342 | 473 | 257 | |
Combined | 2751 | 845 | 441 | 2394 | 605 | 330 | |
CMC | Biological replicate 1 | 9536 | 1958 | 927 | 7380 | 1111 | 594 |
Biological replicate 2 | 7223 | 1760 | 845 | 5509 | 1017 | 538 | |
Combined | 16759 | 2420 | 1116 | 12889 | 1267 | 671 | |
Total | 1450 | 760 | |||||