Comparison of three next-generation sequencing platforms for metagenomic sequencing and identification of pathogens in blood

Table 4 Cross-platform Comparison of Read Mapping to B. anthracis Sterne

Platform	#Replicates	Reads mapped (low)	Reads mapped (med)	Reads mapped (high)	Fraction of reference covered^a	Detected by qPCR?
PGM	2	29,534/15,676	7,689/4,286	247/178	.12/.09	Y
Roche-454	2	384/376	249/240	65/56	.01/.01	Y
MiSeq	2	10,415/41,242	3,024/9,985	1,633/7,930	.07/.19	Y

a: Fraction of reference covered using high stringency mapping parameters as defined in Methods.

ISSN: 1471-2164