Table 1 Genes targeted and primers used for quantification of bovine and A. phagocytophilum DNA
Organism | Gene targeted | Primer Sequences 5' - > 3' | Amplicon size (bp) | Reference |
|---|---|---|---|---|
A. phagocytophilum | ankA | F: CTATCATCCTTGGGTAGTGGCCT | 223 | This study |
R: CTTCTGGTGTTCTCGGACCTTC | ||||
gltA | F: ATATCTACCGGAACCCCCATAGC | 209 | ||
R: AAAAGCTATGACCCTAGAGCGCGT | ||||
groEL | F: GTGATTCGCACGCTCTTAGCAGT | 172 | ||
R: GCCTTCAAGTTGCTGCTGTAAA | ||||
Bos taurus | gapdh | F: TTCACACTCTCCTTCCAGGTACG | 217 | [30] |
R: TCAGGGCCTTAGAGATGGAAA | ||||
ywhaz | F: TACTCCGGACACAGGTAAGGGAC | 212 | ||
R: AGAACCCAGCGGAAGAGAAG | ||||
ppia | F: CTGTTCTAGGTTGGATGGCAAGC | 137 | ||
R: TTTGTCCACAGTCAGCAATGGTGA |