Figure 3

Usage of TRAV/TRDV subgroups in the expressed TRA and TRD chain repertoires. cDNA from CD3⁺γδ^- (αβ T cells) and CD3⁺γδ⁺ (γδ T cells) populations isolated from the PBMC of 2 animals were subjected to unbiased SMART TRAC⁺/ TRDC⁺ PCR amplification and representative amplicons sequenced. The subgroup of the TRAV/TRDV gene expressed by each TRA and TRD chain sequence that was predicted from in silico analysis to be functional (for TRA transcripts n = 60 and 61 and for TRD transcripts n = 68 and 63 for Animals A and B respectively) was identified. The percentage of the TRA transcripts (A) and TRD transcripts (B) that utilised genes from the different TRAV/TRDV subgroups is shown (grey and black bars) alongside the percentage of the genes in the genomic repertoire available for TRA and TRD rearrangement within each TRAV/TRDV subgroup (striped bars).