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Table 4 Two differentially expressed genes (Calcr, top table, and Adh7, bottom table) that are NOT detected by XBSeq, showing the exonic, non-exonic, and predicted true signals (estimated by using Eq.3), from 3 biologial replicates for each WT and MYC mouse tissue.

From: Differential expression analysis of RNA sequencing data by incorporating non-exonic mapped reads

 

Read counts of gene Calcr

  
 

WT (3 replicates)

MYC (3 replicates)

  
 

1

2

3

μ

σ

1

2

3

μ

σ

FC 1

p 2

Exonic

22

4

20

15.3

9.9

68

68

64

66.7

2.3

3.3

0

Non-exonic

64

4

64

44.0

34.6

193

218

199

203.3

13.1

  

Predicted

true signal

0

0

0

0

0

0

0

0

0

0

0

1

 

Read counts of gene Adh7

  
 

WT (3 replicates)

MYC (3 replicates)

  
 

1

2

3

μ

σ

1

2

3

μ

σ

FC 1

p 2

Exonic

633

438

429

500.0

115.3

827

1149

708

894.7

228.2

1.5

0.01

Non-exonic

430

241

322

331.0

95

643

771

513

642.3

129.0

  

Predicted

true signal

203

197

107

169.0

53.8

184

378

195

252.3

109.0

1.2

0.53

  1. 1) FC denotes the fold-change of MYC vs WT, and 2) is the p-value from edgeR-robust (for Exonic read only) and XBSeq (for predicted true signal). Calcr was detected by all other four methods and Adh7 was detected by three methods except DESeq (p-value = 0.06).