Figure 1

Electrophoresis and Southern blot analysis of PEVE Line M – marker, 1 kb ladder (Gibco BRL); Line 1 – denaturing Southern blot hybridization of asexual planarian DNA with ORF2S as a probe. Equivalent data were obtained for all other PEVE clones; Line 2 – denaturing Southern blot hybridization of sexual planarian DNA with ORF2S as a probe. Equivalent data were obtained for all other PEVE clones; Line 3 – non-denaturing Southern blot hybridization of asexual planarian DNA, using the ORF1L fragment as a probe. Similar results were observed with all probes from the Ul PEVE region; Line 4 – non-denaturing Southern blot hybridization of asexual planarian DNA with ORF2S as a probe. Comparable results were obtained with the ORF1S probe; Line 5 – partially purified PEVE DNA on an agarose gel stained with ethidium bromide.