Figure 2

Dot-matrix plots showing level of sequence conservation as a function of residue position in midasin The amino acid sequence of midasin in yeast, Saccharomyces cerevisiae (vertical) is compared with those in fission yeast, Schizosaccharomyces pombe (horizontal, left) and in humans (horizontal, right). Closely spaced dots forming a diagonal line indicate well-conserved regions of sequence. Horizontal dashed lines have been added to indicate the abrupt changes in conservation level that correspond to putative domain boundaries. The off-diagonal lines in the AAA domain correspond to sequence similarity between the six AAA protomers comprising this domain. The rectangular area containing numerous off-diagonal dots in the D/E-rich domain derives from the conserved high percentage of aspartate and glutamate residues in this domain, although the actual sequences are only moderately conserved. Note the short region of relatively well-conserved sequence near the middle of the otherwise less conserved linker domain. The matrices were generated with a stringency of 26% in a sliding window of 40 residues. Abbreviations: Sacce, Saccharomyces cerevisiae; pombe, Schizosaccharomyces pombe.