Table 9 The effect of the amount of template BC2 total RNA on the fidelity, reproducibility and yield of amplification.a
Input total RNA | 3 μg | 1 μg | 300 ng | 100 ng | 30 ng | 10 ng | |
|---|---|---|---|---|---|---|---|
T7 primer used (μg) | 0.5 | 0.2 | 0.1 | 0.1 | 0.1 | 0.1 | |
Average correlation coefficient | Amplified vs. gold standard (fidelity) | 0.80 ± 0.04 (n = 4) | 0.81 ± 0.05 (n = 3) | 0.84 ± 0.05 (n = 2) | ND | ND | ND |
Amplified vs. amplified (reproducibility) | 0.92 ± 0.04 (n = 4) | 0.88 ± 0.04 (n = 3) | 0.90 (n = 2) | ND | ND | ND | |
Yield (μg) | 15.2 ± 5.6 (n = 4) | 7.4 ± 4.6 (n = 3) | 3.1 ± 1.6 (n = 2) | 0.60 ± 0.3 (n = 2) | 0.33 ± 0.2 (n = 3) | 0.11 ± 0.1 (n = 3) | |
Fold of amplification | 507 ± 186 | 740 ± 460 | 1033 ± 530 | 600 ± 300 | 1100 ± 667 | 1100 ± 1000 | |