Figure 6

A: Neighbour-joining tree illustrating the relationship between A. gambiae and D. melanogaster epsilon class GSTs. ClustalW was used to align the putative amino acid sequences of the 8 epsilon class GST subunits in A. gambiae with the 14 putative epsilon class GSTs from D. melanogaster and with GSTS1 from D. melanogaster (as an outgroup). The tree was constructed by the neighbour-joining method from a similarity matrix of pairwise comparisons made using the Jukes-Cantor algorithm. Nodes with distance bootstrap values (500 replicates) of >70% are marked by *. B, Epsilon class GST arrangement in A. gambiae and D. melanogaster. Arrows mark directions of transcription. The solid bars denote the genes and the vertical lines within these mark the approximate introns position.