Refined physical map of the human PAX2/HOX11/NFKB2 cancer gene region at 10q24 and relocalization of the HPV6AI1 viral integration site to 14q13.3-q21.1

Table 2 PCR primer sequences and reaction conditions used to verify localization of HPV6AI1

Primer ID	Locus	Primer Sequence (5' > 3')^a	Annealing Temp (°C)	Mg++ (mM)	Primer Pair ID^b
HPV6apr1	HPV6AI1^c	F-TTATAGATTCGGGGGTACACACGTAG	60	1.5	A
HPV6aRev	HPV6AI1^c	R-AGCAGAGAAAAGGAATGCCGATAT	60	1.5
Chr14gapF	14q13^d	F-TCTTGTTAAATTGTTTGAGTTCC	60	1.5	B
Chr14gapR	14q13^d	R-CTATTCTAATCAAAACAGCATGG	60	1.5
Chr14endF	14q13^d	F-TATTGAATAGAGAGTCCTTTCCC	60	1.5	C
Chr14endR	14q13^d	R-TAAACCTCATCTTGAATTGTAGC	60	1.5
A51	Alu^e	F-GGCGCGGTGGCTCACG	65	1.5	Alu
Sb2ins2	Alu Sb2^f	R-GGCCGGACTGCGGACTGCA	65	1.5

a, F = forward, R = reverse; b, as represented in Figure 3; c,d derived from NCBI Accession No X77607 and NT_025892.8, respectively; e,f derived from Alu-repeat consensus and AluYb8 (Sb2) subfamily-specific consensus sequences, respectively (Labuda et al, unpublished).

ISSN: 1471-2164