Systematic analysis of T7 RNA polymerase based in vitro linear RNA amplification for use in microarray experiments

Table 3 Experimental design of microarray hybridizations

Array Nr.	Cy3 RNA source*	Cy5 RNA source*	Array Nr.	Cy3 RNA source*	Cy5 RNA source*	Array Nr.	Cy3 RNA source*	Cy5 RNA source*
1	b	a	24	a	A2	47	B1	B2
2	b	a	25	b	B1	48	B1	B3
3	c	b	26	b	B1	49	B1	B4
4	c	b	27	b	B2	50	d	pla
5	ref2	pla	28	b	B2	51	d	pla
6	ref2	pla	29	a	A3	52	d	pla
7	ref2	pla	30	a	A3	53	d	pla
8	ref2	d	31	a	A4	54	D	PLA
9	ref2	d	32	b	B3	55	D	PLA
10	ref2	d	33	b	B3	56	D	PLA
11	B1	A1	34	b	B4	57	D	PLA
12	B1	A1	35	b	B4	58	REF1	A1
13	C4	B4	36	A1	A1	59	REF1	A2
14	C4	B4	37	A2	A2	60	REF1	A3
15	REF2	PLA	38	A3	A3	61	REF1	A4
16	REF2	PLA	39	A4	A4	62	REF1	A1
17	REF2	PLA	40	B1	B1	63	REF1	A1
18	REF2	D	41	B2	B2	64	REF1	A2
19	REF2	D	42	B3	B3	65	REF1	A2
20	REF2	D	43	B4	B4	66	REF1	A3
21	a	A1	44	A1	A2	67	REF1	A3
22	a	A1	45	A1	A3	68	REF1	A4
23	a	A2	46	A1	A4	69	REF1	A4

* lowercase letters: total RNA; capital letters: amplified RNA. A, a, B, b, C, c: RNA from human (untreated) mcf7 cell tumours induced in different SCID mice; D, d: adriamycin-treated mcf7 cell tumours grown in a SCID mouse. PLA, pla: human placenta; REF1, ref1, REF2, ref2: different pools of a, b and c.

ISSN: 1471-2164