Figure 2
From: Expression profile of genes regulated by activity of the Na-H exchanger NHE1
Genes differentially regulated in LAPE cells grouped as functioning in growth factor signaling and transcriptional regulation. A. Schematic diagram of growth factor signaling and transcriptional regulation. Red indicates genes with increased expression in LAPE cells compared with LAPN cells, and blue indicates genes with decreased expression. B. Immunoblot analysis of the indicated proteins confirmed increased protein expression in LAPE cells predicted by GeneChip data. C. Relative RT-PCR for the transcription factor C/EBP delta confirmed GeneChip data of increased expression in LAPE cells compared with LAPN cells. D. TaqMan analysis confirmed increased expression of GADD153 in LAPE cells compared with LAPN cells. Data in A represent the means of fold-increase or – decrease in LAPE cells (p < 0.05, n = 5). Data in B, C, and D are representative of 2 to 3 separate cell preparations.