Figure 3
From: Selection and validation of endogenous reference genes using a high throughput approach
Relative expression of gp100, Rho-C and DICE in melanocytic and non-melanocytic samples. Test samples (Cy3) and endogenous reference genes (Cy5) were hybridized to the transcriptome array as described in Figure 1. Test gene / endogenous reference gene Log2 ratios are displayed as a bar graph for NEED8, β-actin and for the averages of test / endogenous reference geneLog2 ratios when the three endogenous reference genes with the best discriminating power in separating gp100 expression between melanocytic (maroon bar) and non-melanocytic (green bar) lesions (NEDD8, RAB3 and FZD6) were used. Samples derived from melanoma metastases are shown by the orange bar. In the boxes the Log10 p2-value of significance of differences between relevant samples (se text) are shown; ns = non significant).