Figure 4

Correlation of gp100 expression estimated obtained by intracellular FACS analysis and by gene profiling with the transcriptome array. Cell lines with different level of gp100 expression by intra-cellular FACS analysis using the gp100-specific mAb HMB45 [35] were used for the analysis. The compiled information from the transcriptome array in which cDNAs from each cell line was spotted are shown. The Cy5/Cy3 (endogenous reference gene / gp100) _Log2 ratios are shown for each endogenous reference gene evaluated. In addition, ratios derived by using the data from all the endogenous reference genes, the best 5 and best 3 according to figure 2 are also presented. Pearson's correlation for the data set is shown for each endogenous reference gene and the same information is summarized graphically at the bottom of the figure. MEF = mean equivalent of fluorescence [35].