Table 1 Process steps The process fail points for cDNA based microarray production. Steps shown in italics are accessible to the testing methods outlined here. Earlier steps may require sequencing to test. The process ID is used to identify steps where the problems, if any, are found.
From: A method for detecting and correcting feature misidentification on expression microarrays
Process ID | Problem Type | Note |
|---|---|---|
-999 | Unidentified | |
0.0 | Source.General | persists across all arrays at clone, 96 level |
0.1 | Source.Contamination | |
0.2 | Source.MisID | |
1.0 | Prep.General | persists across all arrays at clone, 96 level |
1.1 | Prep.Contamination | |
1.2 | Prep.MisID | |
1.2.1 | Prep.MisID.OrderError | |
1.2.2 | Prep.MisID.Rot | |
1.3 | Prep.Fail | |
2.0 | PCR.General | persists for 1 PCR round at 96 level |
2.1 | PCR.Contamination | |
2.2 | PCR.MisID | |
2.3 | PCR.Fail | |
2.4 | PCR.Cleanup | |
3.0 | Replate.General | persists for 1 PCR round at 96 level |
3.1 | Replate.Contamination | |
3.2 | Replate.MisID | |
3.2.1 | Replate.MisID.OrderError | |
3.2.2 | Replate.MisID.96Rot | |
3.2.3 | Replate.MisID.384Rot | |
4.0 | Print.General | persists for 1 print run batch at 384 level |
4.1 | Print.Contamination | |
4.2 | Print.MisID | |
4.2.1 | Print.MisID.OrderError | |
4.2.2 | Print.MisID.Rot | |
4.3 | Print.Fail | dried out plate, too concentrated, too weak etc. |
5.0 | Scan.General | anything after array production |