Figure 2
From: Strengths and weaknesses of EST-based prediction of tissue-specific alternative splicing
RT-PCR validation experiment of a putative brain-specific isoform ( LMO7 ). (A) The additional exon is detected in all tissues (primers F1, R1). (B) The primer pair F1-R2 located on exons flanking the extra exon results in two products where the shorter one is observed in brain, testis and eye (weak band). The predicted brain-specific expression pattern is, in fact, not specific.