Figure 2

Gene discovery is increased by selection of weakly expressed cDNAs clones from a cDNA library made from immature cotyledons. (A) Phosphorimager pattern: A high density membrane containing 18,432 double spotted colonies from the Gm-c1007 cDNA library made from immature cotyledons was hybridized with ³³P-labeled cDNAs transcribed from mRNAs isolated from immature cotyledons. (B) Graphical representation of the new cDNAs selected by the normalization process using filter hybridization. Circles represent the 931 total sequences obtained from the non-normalized source cDNA library Gm-c1007 versus the 1799 sequences of Gm-r1030 that were selected as weakly expressed sequences from the filter hybridization experiments shown in part (A). The intersection of the circles represent sequences common to both sets. H = number of sequences with hits in the databases; N = number of sequences that did not have a hit in the databases; and T = total number of sequences.