Figure 4

Analysis of aRNA purification. (A) Amplified RNA yields and 260/280 ratios with 4 methods of purification. Col – column; PCI – phenol/chlorform/isoamyl alchohol; LiCl – 2.5 M LiCl; G-P – guanidinium-phenol. (B) 1% denaturing agarose/formamide gel of MCF-7 aRNA purified using column (C) Agilent Bioanalyzer analysis of aRNA (from MCF-7) purified by G-P. 6000 nano marker from Ambion (blue) superimposed on the RNA trace. (D) Plot of aRNA yield in μg for different starting total RNA quantities. C1 – OCUB-M with Col.; L1 – OCUB-M with LiCl; G1 – OCUB-M with G-P; C2 – MCF-7 with Col.; L2 – MCF-7 with LiCl; G2 – MCF-7 with G-P.