Figure 4

Identification of domains that affect cIRK1 expression (A) Constructs used in in vitro promoter assays. The putative TATA box, an NF-Y binding site, and an E box are shown in black, hatched and lined boxes, respectively. Numbers in parentheses indicate the length in bp of the promoter region/exon 1 included in each test plasmid. The transcription initiation site is marked with an asterisk. Luc, luciferase reporter gene. (B) In vitro transcription analysis. Plasmid constructs were transiently transfected into DF1 (left) or Qm7 cells (right). Results are shown as fold increases compared with the basal luciferase activity from cells transfected with control pGL-3B vector. Standard errors are shown as bars. *; p value < 0.05, **; p < 0.005.