Homopolymer tract length dependent enrichments in functional regions of 27 eukaryotes and their novel dependence on the organism DNA (G+C)% composition
© Zhou et al; licensee BioMed Central Ltd. 2004
Received: 22 February 2004
Accepted: 14 December 2004
Published: 14 December 2004
DNA homopolymer tracts, poly(dA).poly(dT) and poly(dG).poly(dC), are the simplest of simple sequence repeats. Homopolymer tracts have been systematically examined in the coding, intron and flanking regions of a limited number of eukaryotes. As the number of DNA sequences publicly available increases, the representation (over and under) of homopolymer tracts of different lengths in these regions of different genomes can be compared.
We carried out a survey of the extent of homopolymer tract over-representation (enrichment) and over-proportional length distribution (above expected length) primarily in the single gene documents, but including some whole chromosomes of 27 eukaryotics across the (G+C)% composition range from 20 – 60%. A total of 5.2 × 107 bases from 15,560 cleaned (redundancy removed) sequence documents were analyzed. Calculated frequencies of non-overlapping long homopolymer tracts were found over-represented in non-coding sequences of eukaryotes. Long poly(dA).poly(dT) tracts demonstrated an exponential increase with tract length compared to predicted frequencies. A novel negative slope was observed for all eukaryotes between their (G+C)% composition and the threshold length N where poly(dA).poly(dT) tracts exhibited over-representation and a corresponding positive slope was observed for poly(dG).poly(dC) tracts. Tract size thresholds where over-representation of tracts in different eukaryotes began to occur was between 4 – 11 bp depending upon the organism (G+C)% composition. The higher the GC%, the lower the threshold N value was for poly(dA).poly(dT) tracts, meaning that the over-representation happens at relatively lower tract length in more GC-rich surrounding sequence. We also observed a novel relationship between the highest over-representations, as well as lengths of homopolymer tracts in excess of their random occurrence expected maximum lengths.
We discuss how our novel tract over-representation observations can be accounted for by a few models. A likely model for poly(dA).poly(dT) tract over-representation involves the known insertion into genomes of DNA synthesized from retroviral mRNAs containing 3' polyA tails. A proposed model that can account for a number of our observed results, concerns the origin of the isochore nature of eukaryotic genomes via a non-equilibrium GC% dependent mutation rate mechanism. Our data also suggest that tract lengthening via slip strand replication is not governed by a simple thermodynamic loop energy model.
DNA homopolymer tracts are the simplest of simple sequence repeats (SSRs); the two types being poly(dA).poly(dT) and poly(dG).poly(dC). They are present in all genomes, but in some eukaryotes they are found at high frequencies indicating that the tracts are highly enriched relative to their random occurrence within a random sequence DNA genome of similar base composition. Homopolymer tracts were previously examined systematically in the coding, intron and flanking regions of the slime mold D. discoideum . Only long (N>10 bp) homopolymer tracts were observed at high frequencies in this AT-rich genome. The non-coding regions were found to be highly over-represented in the large poly(dA).poly(dT) tracts compared to random sequences of equivalent base composition containing tracts at frequencies expected for random occurrence. At shorter sequence lengths (2 bp<N<6 bp), poly(dG).poly(dC) tracts were over-represented somewhat more than poly(dA).poly(dT) tracts of comparable length.
Although the elongation of SSR tracts may be due to more than one mechanism , most often the phenomenon has been attributed to slip-strand replication errors, which occur from the slippage and re-annealing of the nascent strand during DNA replication [3–5]. This is a type of mutation that can be affected by the proofreading function of DNA polymerases [6–8]. For example, it has been shown that the proofreading and repair function for DNA polymerase epsilon is efficient for short homopolymer tracts, but that only the mismatch repair system can prevent frameshift mutations in tracts of length 8 nucleotides or greater . These slip-strand errors, which lead to the formation of longer homopolymer tracts, can have deleterious effects. In coding regions, the mutation can cause frame-shift errors, leading to transcription errors and aberrant protein translation. As would be expected from the triplet codon constraints, there appears to be selection against long homopolymer tracts in coding regions. Marx et al  demonstrated that long homopolymer tracts were not present at frequencies higher than expected in the coding regions of D. discoideum DNA.
Compared to nonhomopolymer random B-DNA sequences, poly(dA).poly(dT) tracts have a shorter turn, a smaller axial rise, a narrower and deeper minor groove , a wider and shallower major groove, and are straighter and more rigid over longer lengths. These characteristics are due to the high propeller twist of the base pairs , the maximal overlap/stacking between bases on the same strand, and non-Watson-Crick cross-strand H-bonds between base pairs [9–13]. The result is that long tracts tend to be energetically excluded from nucleosomes [14, 15]. In some definitive studies, it was first shown that tracts of critical and longer lengths are excluded from the reconstituted nucleosome . Other investigators, using native nucleosomes derived from native chicken chromatin, demonstrated that long poly(dA).poly(dT) tracts are excluded from the central core regions . This conclusion received confirmation from a study of the GenBank sequences of D. discoideum, where it was demonstrated that long poly(dA).poly(dT) tracts (N>10 bp) are preferentially spaced at sequence lengths corresponding to the average nucleosome DNA spacing in D. discoideum nucleosomes . In this study, adjacent long poly(dA).poly(dT) tracts, combined with their short nonhomopolymer spacer sequences, exhibited average total lengths that correspond to D. discoideum nucleosomal linker lengths, suggesting their in vivo localization in these chromatin regions and avoidance of the nucleosomal core regions.
Certain natural DNA sequences possess tertiary structures exhibiting a significant amount of curvature that is associated with short homopolymer lengths (4 – 6 bp) of poly(dA).poly(dT) [19, 20]. Also, bending occurs at the junction of these and nonhomopolymer tracts . When short bent poly(dA).poly(dT) tracts are distributed 10 bp apart, they produce additive long range in-plane bending in the axis of the DNA helix [20, 21]. The exact molecular mechanism of this bending behavior is still the subject of considerable experimentation and speculation . DNA bending patterns resulting from spaced poly(dA).poly(dT) tracts have been shown to occur in replication origins and in transcriptional regulatory regions, where a bent configuration is required for activity [23–25].
Poly(dG).poly(dC) tracts form an A-form double-helix. In contrast to poly(dA).poly(dT), the minor groove of these tracts is broad and shallow, while the major groove is deep. But, as with poly(dA).poly(dT) tracts, the tracts are rigid, which leads to the energetic exclusion of poly(dG).poly(dC) from nucleosomes [26, 27]. These characteristics are due to the overlap of adjacent guanine bases and the invariant roll angle between them .
Beyond their structural properties, studies of homopolymer tracts have revealed some biological functions. The poly(dA).poly(dT) tracts can serve as protein binding sites , particularly as upstream promoter elements in the initiation of transcription [30–32] and in recombination . And poly(dG).poly(dC) tracts have been found in certain eukaryotic promoter regions where they are postulated to form 4 stranded G-quartet structures .
As the number of DNA sequences publicly available increases, the representation (over and under) of homopolymer tracts in different genomes can be compared. Qualitative comparisons have been made between five eukaryotic and two prokaryotic genomes: P. falciparum (also very AT-rich), H. sapiens, S. cerevisiae, C. elegans, A. thaliana, E. coli and M. tuberculosis . As with D. discoideum (1), it was shown in that study that homopolymer tracts occur in the non-coding regions at over-represented frequencies for poly(dA).poly(dT). Poly(dG).poly(dC) tracts were found to be over-represented in some but not all organisms at short lengths. However, over-representation was observed only in the eukaryotic genomes, not the prokaryotes.
In the present study, we have carried out a broad survey of non-overlapping homopolymer tract frequencies in the genomic sequences of 27 eukaryotic organisms across the base composition range from 20–60% (G+C). Within the coding, intron and flanking DNA functional compartments of largely single copy genes from these organisms, we compared the observed poly(dA).poly(dT) and poly(dG).poly(dC) tract frequencies in two size ranges to the tract occurrence frequencies expected for random tract occurrence in DNA compartments of the same base compositions. A large fraction of the 27 eukaryotes exhibited significant over-representations (enrichment) of longer length (N ≥ 9 bp) poly(dA) and poly(dT) tracts in their intron and flanking sequences, but not their coding sequences. This occurred in a novel base composition dependent fashion. A much smaller number of the 27 organisms exhibited significant over-representations of longer length (N ≥ 9) poly(dG) and poly(dC) tracts. For P. falciparum and S. cerevisiae single gene containing sequences as well as whole chromosomes, all homopolymer tracts were found to have similar length dependent frequencies and therefore over-representation in their functional compartments.
Summary of the sequence files of the 27 organisms studied
DOCUMENTS BEFORE CLEAN-UP
DOCUMENTS AFTER CLEAN-UP
chromosome II, III
The (G+C)% in different compartments of the 27 organisms
Long homopolymer tracts are over-represented in non-coding sequences of AT-rich eukaryotes
From Figure 1A, it is clear that the frequency of poly(dA).poly(dT) tracts in the very AT-rich Pf flanking regions are much higher than Dm and Nc tract frequencies. This becomes more pronounced when the tract length N becomes larger (N ≥ 7 bp) and reaches a maximum at around N ≥ 10 bp. On the other hand, the frequencies of poly(dG) or poly(dC) tracts in Pf are significantly lower and no differential N dependence is observed. Meanwhile, no significant difference can be observed between Dm and Nc, except for the higher values at longer N in Nc sequences. Similar behavior is also observed in intron sequences in Figure 1B. The over-representation of poly(dA) or poly(dT) tracts in intron regions is also evident at higher N values. However, this behavior is almost non-existent in coding regions (Figure 1C), except for Pf, which is the most AT-rich eukaryote in all the 27 of our survey. This organism exhibited over-representation of poly(dA) tracts in its coding region as well as poly(dT) tracts as we shall see below.
It is also very clear that the longer poly(dA) and poly(dT) tracts, usually of length larger than 20 bp, are only detected in flanking regions. In all cases, the plotted curves exhibited a transition region of changing slope, points falling between tract lengths 6 bp and 9 bp. This behavior, as we have described previously in D. discoideum DNA, leads one to conclude that the long poly(dA) and poly(dT) tracts are over-represented relative to random tract occurrence in random DNA sequences of equivalent base composition . This is a fact that we illustrate and quantitate later in this study. By contrast, the nearly linear relationship of points in Figure 1, for all the organisms' tracts of all types at lengths N ≤ 6 bp indicates a similarity that differs for each organism only by the individual linear relationships being offset from each other. This is a trivial consequence of the different base compositions of the DNAs, giving rise to frequencies of any given tract at levels near those expected based on random occurrence in that base composition.
Comparing tract frequencies from single genes with those from whole chromosomes
Quantitating the over-representation of tracts
For N ≥ 9 bp tracts in coding sequences (Figure 5A &5B), there were not enough tracts to allow calculation of f slope values from the Figure 1 type data. However, in flanking and intron sequences (Figure 3A and Figure 4A), the ratio was determined and is much higher than 1 for all the organisms, indicating that the poly(dA).poly(dT) tracts are significantly over-represented. The behavior of poly(dA).poly(dT) tracts in both flanking and intron sequences are similar and demonstrate a novel and interesting dependence of over-representation on the base composition of the organism's DNA. Starting at 30 %(G+C) and increasing to 50 %(G+C) (note linear fit trend line), these tracts are increasingly over-represented as the ratio trends from around 1.5 up to 4.0.
For lengths N ≥ 9 bp in Figures 3B and 4B, we observed similar behavior for poly(dG).poly(dC) tracts. In a few organisms between 35%–50% (G+C), there is a high ratio of frequencies indicating a high level of over-representation. However, longer poly(dG).poly(dC) tracts are not over-represented and do not occur at long lengths as we show later in nearly as many organisms as we observed for poly(dA).poly(dT) tracts. Therefore, even though there appears to be evidence for a trend in these figures, we have not indicated with a negative slope linear trend line, mirror-image trend behavior to that exhibited by the poly(dA).poly(dT) tracts.
The over-representation of long poly(dA).poly(dT) tracts exhibit exponential frequency increases compared to predicted values
Poly(dA).poly(dT) tracts show inverse correlation between (G+C)% composition and threshold value
For homopolymer tracts of each type in coding, intron and flanking DNAs, data of the type shown for poly(dA) tracts in Figure 7A were calculated and the linear fit slopes are presented in Figure 7 panels B-D, respectively. For all the data, the poly(dA).poly(dT) tracts exhibit uniformly negative slopes between -4 and -10, while the poly(dG).poly(dC) tracts all exhibit positive slopes between 4.5 and 7. In coding sequences, poly(dA) tracts showed a sharp drop between 0.7 and 0.8 while poly(dT) tracts exhibited a slow increase. No poly(dG) or poly(dC) tracts of significant length occur in coding DNA, which did not allow over-representation to be exhibited at these threshold values. Therefore, no slope points are shown. For intron sequences in panel C, poly(dG).poly(dC) tracts exhibited no significant consistent slope trend. However, poly(dC) tracts have overall greater slopes than poly(dG) tracts. Likewise for poly(dA).poly(dT) tracts, no trend is evident but the latter possesses significantly greater slopes than the former. For flanking sequences, all four tract types exhibited increasing slopes as threshold values increased. As was true for intron sequences, poly(dC) again had overall somewhat greater slopes than poly(dG) tracts. Similar behavior was observed for poly(dA).poly(dT) tracts in both intron and flanking DNA sequence types, with poly(dA) again occurring at greater slopes than poly(dT).
The highest over-representation and over-proportional length of homopolymer tracts appear in median GC% organisms
By contrast, the average behavior of intron and flanking regions in Figure 8 is that tracts of all types, but especially poly(dA).poly(dT) tracts, occur at significantly over-proportional lengths. This fact is consistent with their significant over-representation levels that we previously presented in Figures 3,4,5. Very long poly(dA).poly(dT) tracts are observed in non-coding regions of some organisms, at lengths greater than 20 bp in excess of the expected length. Interestingly, the highest over-representation levels of tracts are found in organisms between 30% – 50 % (G+C) base composition. The only exception to this was found in Dd, the most AT-rich organism we studied, where the longest poly(dA) tracts were 71 bp. Higher poly(dG).poly(dC) tract frequencies than expected for organisms greater than 40 % (G+C) base composition were observed in Figure 3B and Figure 4B. The same was true for the most AT-rich ones – Dd and Pf. Figure 8 panels B and C correspondingly exhibit significant levels of over-proportional lengths of poly(dG).poly(dC) tracts, consistent with over-representation, for organisms greater than 30% (G+C) base composition and exhibit moderate over-representation of poly(dG).poly(dC) tracts for Dd and Pf.
As a result of recent progress in the rate of DNA sequencing, the amount of sequenced DNA from many organisms has grown significantly. This has allowed our systematic study of the behavior of non-overlapping homopolymer tract frequencies in the 27 eukaryotes in this study spanning the 20% – 60 % (G+C) base composition range. Pre-processing of each of the 27 eukaryotes' largely single gene containing sequence files eliminated sequence redundancies that would introduce bias into the frequency calculations [39–41], that would not be representative of the biological genomes. In most organisms, well over 10% of the documents obtained were judged to contain redundant sequences and were removed by the CleanUP program (Table 1).
From our results in this study, it is clear that long homopolymer tracts are over-represented in non-coding sequences, but not coding sequences, within eukaryotic genomes of all base compositions. This is perhaps not surprising considering that the coding sequence populations must satisfy the constraints of the triplet genetic code. In addition, organisms might minimize the numbers of tracts in coding regions to avoid the severe, even fatal frame-shift mutations that might be introduced by slippage-replication events at tracts [3–5, 42]. In nearly all the organisms we studied, poly(dA).poly(dT) tracts were very much over-represented, beginning to be significantly enriched at lengths around 4–10 bp. These tracts also occurred at over-proportional lengths. This was particularly the case for organisms between 30% – 50% (G+C) composition, where over-proportional lengths were pronounced. By contrast, poly(dG).poly(dC) tracts, somewhat over-represented, do not occur at over-proportional lengths. This extends the findings of our previous D. discoideum DNA study that first described the tract over-representation transition region occurring at around 8–10 bp for poly(dA).poly(dT) sequences and their high over-proportional lengths . Somewhat similar observations were made in a subsequent study of five eukaryotic organisms . In general studies of repetitive sequences, poly(dA).poly(dT) tracts have been observed to be over-represented within eukaryotic genomes while poly(dG).poly(dC) tracts are significantly rarer [2, 43]. Specific human repetitive sequences, such as the Alu elements, have been shown to contain long poly(dA).poly(dT) tracts, representing a significant repetitive sequence location for some of the over-represented tracts we observed in this study .
It has been suggested in a previous study that the over-representation occurring around 7–10 bp represented the minimum thermodynamic length required for any simple sequence repeat such as homopolymer tracts to undergo expansion by slip strand replication . However, in our current study of 27 eukaryotes of widely varying base composition, we present more extensive results, especially those in Figure 7, that demonstrate this is not the case. Depending upon the threshold tract size value chosen to express over-representation of the tracts, the N value where over-representation occurs for A tracts can be seen in Figure 7A to range for all the organisms from as low as 4–6 bp for 0.3 threshold (2× enrichment) to 8–11 bp for 1.0 threshold (10× enrichment). Furthermore, for poly(dA).poly(dT) tracts, the (G+C)% base composition vs. N slopes are negative for all thresholds, while for poly(dG).poly(dC) tracts the slopes are positive for all thresholds. This means that the base composition of the organism is the most important determinant of the particular threshold N value where over-representation begins and argues against an absolute solely thermodynamic determinant to the N value where over-representation begins via slip strand replication. In fact, our observed negative slopes for the poly(dA) tracts in Figure 7A, means that in higher (G+C)% composition organisms, the poly(dA) tracts become enriched at shorter N values than in (G+C)% poor organisms. This result is counter-intuitive to a thermodynamic argument, since the high (G+C)% base composition in neighboring sequences around a short poly(dA) tract in a high (G+C)% organism would be expected to resist the tract looping out to allow for slip strand replication because of the higher level of base stacking stabilization energy in the (G+C)-rich neighboring sequences. We believe that these (G+C)% composition dependent variable threshold N values we observed here are describing a complex mechanism that determines successful tract lengthening, rather than a single thermodynamic criterion for successful DNA looping during slip strand replication.
The reason why poly(dG).poly(dC) tracts occur only at short lengths in eukaryotes may have to do with some interesting structural and energetic polymorphisms of these sequences. Even short tracts of this type have the ability to rearrange from the right-handed double helix to form G-quadraplex structures. These structures have been implicated in biological function in systems as diverse as eukaryotic immunoglobulin switch regions , telomeric repeats on chromosome ends  and promoter regions . Therefore, eukaryotes may select against these tracts at any significant length in order to minimize problems resulting from the significant structural plasticity of these tracts. Another potential problem with these tracts is the fact that they represent potential reservoirs of oxidative damage. Recently, long-range electron transfer has been demonstrated to occur through the delocalized molecular orbitals of the stacked bases in the DNA double helix . The electron transfer energy in these studies is insensitive to distance along the helix, but is sensitive to the level of base stacking. Therefore, these electron transfer events ultimately cause oxidative damage at GG dinucleotides, a base pair doublet that has high stacking levels. Even greater intensities of photo-damage were observed for GGG triplets. Therefore, eukaryotic organisms have a second compelling reason to mostly avoid the use of these homopolymer tracts at any significant length-a fact reflected in the data we have presented here.
It must be kept in mind in these discussions of homopolymer tract over-representation, that these tracts represent only a subset of the larger sequence class of polypurines and polypyrimidines that exist in and are over-represented within all eukaryotes. In a study of over 700 sequenced chromosomes or long sequences contained in plasmids , a bias toward longer polypurine and polypyrimidine tracts in eukaryotes was reported as a function of length N, similar to the homopolymer poly(dA).poly(dT) tract frequency behavior we have reported here.
We have previously observed that the long (N>10 bp) poly(dA).poly(dT) tracts over-represented in D. discoideum DNA (1) were not randomly distributed within the sequences from that organism. In fact, they are arrayed with an average spacing that corresponds to the repeating nucleosome DNA length found experimentally in D. discoideum chromatin . And in that study, adjacent long pairs of tracts plus the intervening non-tract DNA were found to occur within a length corresponding to the internucleosomal linker DNA size found in D. discoideum chromatin. These results suggest that the long tracts only occur in restricted locations in chromatin. This supposition is supported by more recent experimental studies in D. discoideum chromatin compared to calculations of poly(dA).poly(dT) tract spacings in D. discoideum DNA (Marx, K.A., Zhou, Y. and Kishawi, I. unpublished results). That long poly(dA).poly(dT) tracts avoid being located within nucleosome core regions was experimentally determined from sequencing studies of native chicken erythrocyte chromatin . In agreement with this line of reasoning, recent studies have shown that the nucleosome structure readily incorporates DNA containing short tracts, such as the sequence A5TATA4, but longer tracts such as those found in the sequence A15TATA16, completely disrupt the phasing of nucleosomes . Short tracts not only are incorporated into nucleosomes, but they actually represent more stable than average nucleosome positioning sequences when they occur in-phase with the helical turn at roughly every 10 bp . In human NF1-the Alu repeat element is blocked in vitro from forming a nucleosome by the presence of a bipartite T14A11 tract sequence .
Different investigators have postulated two additional functions for tracts. The first is their use as promoters. This function may be synergistic with the long poly(dA).poly(dT) tracts preventing the formation of nucleosome structures. The second is as DNA binding sequences for specific poly(dA).poly(dT) tract binding proteins that possess some as yet unknown function. There are a number of reports that poly(dA).poly(dT) tracts function in eukaryotes as promoter sequences. In D. discoideum DNA, the actin genes contain a remarkably long (45 bp) promoter upstream of the TATA box . In this study, the length of the tract was shown to correlate with the transcriptional level of these genes. A number of studies have demonstrated similar long tract promoter activity in yeast promoter regions [32, 53, 54] and in various mammalian  and human promoters . In many of these studies, it was demonstrated that the promoter activity of the long tracts was correlated with this sequence being nucleosome free or not complexed with a protein.
In the case of potential tract function where proteins bind to long poly(dA).poly(dT) tracts, there are a few investigated examples. The small protein datin, 13 kD, has been isolated from S. cerevisiae cells . It has a required tract-binding site that is 9–11 bp in length and its function upon tract binding is unknown. Two high affinity poly(dA).poly(dT) tract-binding proteins, 70 and 74 kD species of unknown function, have been identified in D. discoideum . Another example of a tract binding protein has been discovered in D. discoideum, where some 200 copies of terminal repeat retrotransposons are under transcriptional control by a 134 bp DNA control element . Within this control element, a nuclear protein called CMBF binds to two almost homopolymeric 24 bp poly(dA).poly(dT) sequences. This CMBF protein contains so-called 'A.T hook' regions that interact with a 5–6 contiguous A:T base pair tract. These 'A.T hooks' are found in a number of other (A+T)-rich sequence binding proteins such as HMG-I, DAT1 from yeast, D1 from D. melanogaster and human UBF. In summary, it is unclear what functions these various pure poly(dA).poly(dT) tract binding proteins serve, and how their binding occurs at specific tracts while other tracts remain free of protein. The one point that can be stated with certainty is the correlation between tract binding site size (8–11 bp) of the proteins and the upper threshold (8–11 bp) size where tracts become significantly over-represented or enriched in our study. We believe that this similarity is not coincidental and is a consequence of some functional linkage.
A novel aspect of our study was that for both flanking and intron sequences the over-representation of the poly(dA) and poly(dT) tracts were actually more pronounced in less (A+T)-rich organisms as compared to the most (A+T)-rich, Dd and Pf. Also novel was that poly(dA) and poly(dT) tracts showed negative slopes between the organisms' DNA (G+C)% composition and the threshold value. Thus, the higher the (G+C) base composition, the lower the tract length at which over-representation occurs. In fact, the highest over-representations of homopolymer tracts were observed in median (G+C)% organisms from 30–50%. Also, the distribution was almost symmetric with respect to the different organisms' (G+C)%. We believe that these results could be explained as a result of the insertion of retrotransposon elements into DNA. Eukaryotic transposons are known to be a widely occurring class of repeated DNA sequences ranging in size from about 1 kb to 8 kb. They contain inverted sequence repeats at their termini. The most common transposon class is comprised of retrovirus-like transposons , thought to arise from the integration of retroviral RNA sequences into a given eukaryotic genome. The resulting retrotransposon elements do not represent infectious viral DNA and are not transcribed since they lack accompanying promoter sequences. These DNA sequences do possess a poly(dA).poly(dT) tract that resulted from the 3' poly A tail on the original viral mRNA that formed the retrotransposon. Typical retrovirus-like retrotransposons, such as copia in D. melanogaster and IAP in M. musculus, are known to occur in thousands of copies in their respective genomes . Therefore, inserted retrotransposon elements represent the likely origin of the excess over-representation of poly(dA).poly(dT) sequences that we observed in the majority of eukaryotes in this study, irrespective of their overall base composition.
The single copy gene DNA sequences from 27 eukaryotic organisms were retrieved from GenBank, EMBL, and DDBJ, the members of the tripartite, international collaboration of sequence databases . Every search excluded: ESTs (expressed sequence tag), STSs (sequence-tagged sites), and GSSs (genomic survey sequence), and were limited to organism and genomic DNA only. Moreover, sequences designated: "mitochondrion", "chloroplast", and "chromosome" were also excluded in the search query via these keywords using Boolean operators. In addition, the whole chromosome sequences from 2 of these organisms were also retrieved. The eukaryotic organisms covered are tabulated in Table 1.
The GenBank documents were processed by the program "CleanUP", kindly provided by the Department of Biochemistry and Molecular Biology, University of Bari, Italy . Our purpose in using the program was to get rid of redundancy in our sequence collections so that no bias would be introduced into the homopolymer tract distributions we calculated . The settings for the program are: precision factor (0), different adjacent nucleotides (2), threshold similarity percentage for searching (95.0), overlapping percent for searching (50.0), local similarity percent (70.0), percent ambiguous symbols (e.g. N) to skip matches (10), overlapping percent for cleaning (90.0), minimum length for overlapping (1), minimum length for overlapping segment (20), sequence minimum length so that a sequence is processed, otherwise is cleaned (30). This application of "CleanUP" results in eliminating all the sequences less than 30 bp in length, with more than 20 bp overlapping with the primary sequence (the sequence used use as a basis for comparison), and possessing over 90% similarity with the primary sequence.
Then the redundancy cleaned sequence files were input into the "Compile" program. Compile is part of the "MeltSim" program for the Windows suite of applications [37, 63]. This program was used to extract raw sequences from the GenBank-formatted documents. Sequences of the functional categories, coding, intron and flanking were extracted according to their location tags. Respectively, "CDS" is for coding sequences, "intron" is for intron sequences, and "5'UTR", "3'UTR" and any other sequences excluding "CDS" and "intron" are all included into flanking sequences. The sequences were then concatenated into ASCII text files, one each for coding, intron and flanking. The ends of the individual sequences, as they appeared in the individual GenBank-formatted documents, were tagged to prevent the artifactual joining of those sequences that could result in the creation of artifactual long tracts. The basic characteristics of the coding, intron and flanking files that we used as computational start points for homopolymer tract frequency determination are summarized in Table 2.
Each file was subsequently analyzed using the program "Poly" [38, 64], which calculates parameters for non-overlapping homopolymer tracts, including the total base count for each file, GC composition, and the numbers and the frequencies of the homopolymer tracts of different lengths. Poly uses a moving window of 1 bp in length to differentiate tracts and spacers, taking into account the tags used to prevent the artifactual concatenation. These data and additional information are kept as data objects in the program and can be manipulated in various ways.
Poly calculates the observed tract frequency of base i, , of length N by the formula:
Using the relationship:
The level of tract representation for base i is then calculated as the ratio of observed to predicted frequencies, defined as "Representation"(R):
Values larger than 1 indicates base i tracts are "over-represented", while values less than 1 indicate tracts are "under-represented". The log(R) verses N plots presented in Figure 6 were generated using the program Gnuplot [38, 65]. The values of N at 0.3 to 1.0 of the log(R) were found using linear interpolation of the data. We term these N values, thresholds, corresponding to particular enrichments of tract occurrence.
The maximum expected length of a homopolymer tract of base i, , given the base composition of the entire sequence, , is calculated by the formula:
P values larger than 1 are called "over-proportional" and P values less than 1 are "under-proportional". P represents the tract length comparison on the x-axis of Figure 8, which is complementary to the parameter R for tract frequency comparisons on y-axis. They are both important parameters for the evaluation of the frequency and length distributions of any tracts.
- Marx KA, Hess ST, Blake RD: Characteristics of the large (dA).(dT) homopolymer tracts in D. discoideum gene flanking and intron sequences. J Biomol Struct Dyn. 1993, 11: 57-66.View ArticlePubMed
- Toth G, Gaspari Z, Jurka J: Microsatellites in different eukaryotic genomes: survey and analysis. Genome Res. 2000, 10: 967-981. 10.1101/gr.10.7.967.PubMed CentralView ArticlePubMed
- Streisinger G, Okada Y, Emrich J, Newton J, Tsugita A, Terzaghi E, Inouye M: Frameshift mutations and the genetic code. Cold Spring Harb Symp Quant Biol. 1966, 31: 77-84.View ArticlePubMed
- Kunkel TA, Soni A: Mutagenesis by transient misalignment. J Biol Chem. 1988, 263: 14784-14789.PubMed
- Fujii S, Akiyama M, Aoki K, Sugaya Y, Higuchi K, Hiraoka M, Miki Y, Saitoh N, Yoshiyama K, Ihara K, Seki M, Ohtsubo E, Maki H: DNA replication errors produced by the replicative apparatus of Escherichia coli. J Mol Biol. 1999, 289: 835-850. 10.1006/jmbi.1999.2802.View ArticlePubMed
- Strand M, Prolla TA, Liskay RM, Petes TD: Destabilization of tracts of simple repetitive DNA in yeast by mutations affecting DNA mismatch repair. Nature. 1993, 365: 274-276. 10.1038/365274a0.View ArticlePubMed
- Kroutil LC, Register K, Bebenek K, Kunkel TA: Exonucleolytic proofreading during replication of repetitive DNA. Biochemistry. 1996, 35: 1046-1053. 10.1021/bi952178h.View ArticlePubMed
- Tran HT, Keen JD, Kricker M, Resnick MA, Gordenin DA: Hypermutability of homonucleotide runs in mismatch repair and DNA polymerase proofreading yeast mutants. Mol Cell Biol. 1997, 17: 2859-2865.PubMed CentralView ArticlePubMed
- Alexeev DG, Lipanov AA, Skuratovskii I: Poly(dA).poly(dT) is a B-type double helix with a distinctively narrow minor groove. Nature. 1987, 325: 821-823. 10.1038/325821a0.View ArticlePubMed
- Aymami J, Coll M, Frederick CA, Wang AH, Rich A: The propeller DNA conformation of poly(dA).poly(dT). Nucleic Acids Res. 1989, 17: 3229-3245.PubMed CentralView ArticlePubMed
- Arnott S, Chandrasekaran R, Birdsall DL, Leslie AG, Ratliff RL: Left-handed DNA helices. Nature. 1980, 283: 743-745. 10.1038/283743a0.View ArticlePubMed
- Nelson HC, Finch JT, Luisi BF, Klug A: The structure of an oligo(dA).oligo(dT) tract and its biological implications. Nature. 1987, 330: 221-226. 10.1038/330221a0.View ArticlePubMed
- Lipanov AA, Chuprina VP: The structure of poly(dA):poly(dT) in a condensed state and in solution. Nucleic Acids Res. 1987, 15: 5833-5844.PubMed CentralView ArticlePubMed
- Rhodes D: Nucleosome cores reconstituted from poly(dA-dT) and the octamer of histones. Nucleic Acids Res. 1979, 6: 1805-1816.PubMed CentralView ArticlePubMed
- Simpson RT, Kunzler P: Chromatin and core particles formed from the inner histones and synthetic polydeoxyribonucleotides of defined sequence. Nucleic Acids Res. 1979, 6: 1387-1415.PubMed CentralView ArticlePubMed
- Kunkel GR, Martinson HG: Nucleosomes will not form on double-stranded RNA or over poly(dA).poly(dT) tracts in recombinant DNA. Nucleic Acids Res. 1981, 9: 6869-6888.PubMed CentralView ArticlePubMed
- Satchwell SC, Drew HR, Travers AA: Sequence periodicities in chicken nucleosome core DNA. J Mol Biol. 1986, 191: 659-675.View ArticlePubMed
- Marx KA, Hess ST, Blake RD: Alignment of (dA).(dT) homopolymer tracts in gene flanking sequences suggests nucleosomal periodicity in D. discoideum DNA. J Biomol Struct Dyn. 1994, 12: 235-246.View ArticlePubMed
- Marini JC, Levene SD, Crothers DM, Englund PT: A bent helix in kinetoplast DNA. Cold Spring Harb Symp Quant Biol. 1983, 47: 279-283.View ArticlePubMed
- Wu HM, Crothers DM: The locus of sequence-directed and protein-induced DNA bending. Nature. 1984, 308: 509-513. 10.1038/308509a0.View ArticlePubMed
- Koo HS, Wu HM, Crothers DM: DNA bending at adenine.thymine tracts. Nature. 1986, 320: 501-506. 10.1038/320501a0.View ArticlePubMed
- Hagerman PJ: Straightening out the bends in curved DNA. Biochim Biophys Acta. 1992, 1131: 125-132.View ArticlePubMed
- Bossi L, Smith DM: Conformational change in the DNA associated with an unusual promoter mutation in a tRNA operon of Salmonella. Cell. 1984, 39: 643-652. 10.1016/0092-8674(84)90471-9.View ArticlePubMed
- Snyder M, Buchman AR, Davis RW: Bent DNA at a yeast autonomously replicating sequence. Nature. 1986, 324: 87-89. 10.1038/324087a0.View ArticlePubMed
- Zahn K, Blattner FR: Direct evidence for DNA bending at the lambda replication origin. Science. 1987, 236: 416-422.View ArticlePubMed
- Jayasena SD, Behe MJ: Competitive nucleosome reconstitution of polydeoxynucleotides containing oligoguanosine tracts. J Mol Biol. 1989, 208: 297-306. 10.1016/0022-2836(89)90390-2.View ArticlePubMed
- Puhl HL, Gudibande SR, Behe MJ: Poly(dA:dT) and other synthetic polydeoxynucleotides containing oligoadenosine tracts form nucleosomes easily. J Mol Biol. 1991, 222: 1149-1160. 10.1016/0022-2836(91)90598-Z.View ArticlePubMed
- McCall M, Brown T, Kennard O: The crystal structure of d(G-G-G-G-C-C-C-C). A model for poly(dG).poly(dC). J Mol Biol. 1985, 183: 385-396. 10.1016/0022-2836(85)90009-9.View ArticlePubMed
- Travers AA: DNA conformation and protein binding. Annu Rev Biochem. 1989, 58: 427-452. 10.1146/annurev.bi.58.070189.002235.View ArticlePubMed
- Struhl K: Naturally occurring poly(dA-dT) sequences are upstream promoter elements for constitutive transcription in yeast. Proc Natl Acad Sci USA. 1985, 82: 8419-8423.PubMed CentralView ArticlePubMed
- Boettcher B: Transcription initiation and nuclease-sensitive sites upstream of the epsilon-globin gene in K562 cells are related to poly(dA).poly(dT) sequences. J Theor Biol. 1990, 146: 333-339.View ArticlePubMed
- Iyer V, Struhl K: Poly(dA:dT), a ubiquitous promoter element that stimulates transcription via its intrinsic DNA structure. Embo J. 1995, 14: 2570-2579.PubMed CentralPubMed
- Schultes NP, Szostak JW: A poly(dA.dT) tract is a component of the recombination initiation site at the ARG4 locus in Saccharomyces cerevisiae. Mol Cell Biol. 1991, 11: 322-328.PubMed CentralView ArticlePubMed
- Siddiqui-Jain A, Grand CL, Bearss DJ, Hurley LH: Direct evidence for a G-quadruplex in a promoter region and its targeting with a small molecule to repress c_myc transcription. Proc Natl Acad Sci. 2002, 99: 11593-11598. 10.1073/pnas.182256799.PubMed CentralView ArticlePubMed
- Dechering KJ, Cuelenaere K, Konings RNH, Leunissen JAM: Distinct frequency-distributions of homopolymeric DNA tracts in different genomes. Nucleic Acids Res. 1998, 26: 4056-4062. 10.1093/nar/26.17.4056.PubMed CentralView ArticlePubMed
- Attimonelli M, Liuni S, Pesole G: CLEANUP : a fast computer program for removing redundancies from nucleotide sequence database. Comput Appli Biosci. 1996, 12: 1-8.
- Blake RD, Bizzaro JW, Blake JD, Day GR, Delcourt SG, Knowles G, Marx KA, SantaLucia J: Statistical Mechanical Simulation of Polymeric DNA Melting with MELTSIM. Bioinformatics. 1999, 15: 370-375. 10.1093/bioinformatics/15.5.370.View ArticlePubMed
- Bizzaro JW, Marx KA: Poly: a quantitative analysis tool for simple sequence repeat (SSR) tracts in DNA. BMC Bioinformatics. 2003, 4: 22-27. 10.1186/1471-2105-4-22.PubMed CentralView ArticlePubMed
- Lamperti ED, Kittelberger JM, Smith TF, Villa-Komaroff L: Corruption of genomic databases with anomalous sequence. Nucleic Acids Res. 1992, 20: 2741-2747.PubMed CentralView ArticlePubMed
- Paricio N, Terol J, Artero RD, Perez-Alonso M: Poly(dC) segments and cloning artifacts in databases. Genetics. 1995, 139: 487-488.PubMed CentralPubMed
- van Hintum TJL, van Treuren R: Molecular markers: Tools to improve Genebank efficiency. Cell Mol Biol Lett. 2002, 7: 737-744.PubMed
- Kirchner JM, Tran H, Resnick MA: A DNA polymerase ε mutant that specifically causes +1 frameshift mutations within homonucleotide runs in yeast. Genetics. 2000, 155: 1623-1632.PubMed CentralPubMed
- Katti M, Ranjekar K, Gupta VS: Differential Distribution of Simple Sequence Repeats in Eukaryotic Genome Sequences. Mol Biol Evol. 2001, 18: 1161-1167.View ArticlePubMed
- Roy AM, Carroll ML, Nguyen SV, Salem AH, Oldridge M, Wilkie AO, Batzer MA, Deinenger PL: Potential Gene Conversion and Source Genes for Recently Integrated Alu Elements. Genome Res. 2000, 10: 1485-1495. 10.1101/gr.152300.View ArticlePubMed
- Sen D, Gilbert W: Formation of parallel four-stranded complexes by guanine-rich motifs in DNA and its implications for meiosis. Nature. 1988, 334: 364-366. 10.1038/334364a0.View ArticlePubMed
- Sundquist WI, Klug A: Telomeric DNA dimerizes by formation of guanine tetrads between hairpin loops. Nature. 1989, 342: 825-829. 10.1038/342825a0.View ArticlePubMed
- Hall DB, Holmlin RE, Barton JK: Oxidative DNA damage through long-range electron transfer. Nature. 1996, 382: 731-735. 10.1038/382731a0.View ArticlePubMed
- Ussery D, Soumpasis DM, Brunak S, Starfeldt HH, Worning P, Krogh A: Bias of purine streches in sequenced chromosomes. Comput Chem. 2002, 26: 531-541. 10.1016/S0097-8485(02)00013-X.View ArticlePubMed
- Shimizu M, Mori T, Sakurai T, Shindo H: Destabilization of nucleosomes by an unusual DNA conformation adopted by poly(dA).poly(dT) tracts in vivo. Embo J. 2000, 19: 3358-3365. 10.1093/emboj/19.13.3358.PubMed CentralView ArticlePubMed
- Widlund H, Cao H, Simonsson S, Magnusson E, Simonsson T, Nielsen PE, Kahn JD, Crothers DM, Kubista M: Identification and Characterization of Genomic Nucleosome-positioning Sequences. J Mol Biol. 1997, 267: 807-817. 10.1006/jmbi.1997.0916.View ArticlePubMed
- Englander EW, Howard BH: A naturally occurring T14A11 tract blocks nucleosome formation over the human neurofibromatosis type 1 (NF1)-Alu element. J Biol Chem. 1996, 271: 5819-5823. 10.1074/jbc.271.10.5819.View ArticlePubMed
- Hori R, Firtel RA: Identification and Characterization of multiple A/T-rich cis-acting elements that control expression from Dictyostelium actin promoters: the Dictyostelium actin upstream activating sequence confers growth phase expression and has enhancer-like properties. Nucleic Acids Res. 1994, 22: 5099-5111.PubMed CentralView ArticlePubMed
- Suter B, Schnappauf G, Thomas F: Poly(dA:dT) sequences exist as rigid DNA structures in nucleosome-free yeast promoters in vivo. Nucleic Acids Res. 2000, 28: 4083-4089. 10.1093/nar/28.21.4083.PubMed CentralView ArticlePubMed
- Filetici P, Aranda C, Gonzalez A, Ballario P: GCN5, a yeast transcriptional coactivator, induced chromatin reconfiguration of HIS3 promoter in vivo. Biochem Biophys Res Commun. 1998, 242: 84-87. 10.1006/bbrc.1997.7918.View ArticlePubMed
- Koch KA, Thiele DJ: Functional analysis of a homopolymeric (dA-dT) element that provides nucleosome access to yeast and mammalian transcription factors. J Biol Chem. 1999, 274: 23752-23760. 10.1074/jbc.274.34.23752.View ArticlePubMed
- Fashena S, Reeves R, Ruddle NH: A poly(dA:dT) upstream activating sequence binds high-mobility group I protein and contributes to lymphotoxin (tumor necrosis factor-β) gene regulation. Mol Cell Biol. 1992, 12: 894-903.PubMed CentralView ArticlePubMed
- Winter E, Varshavsky A: A DNA binding protein that recognizes oligo(dA).oligo(dT) tracts. Embo J. 1989, 8: 1867-1877.PubMed CentralPubMed
- Garreau H, Williams JG: Two nuclear binding proteins of Dictyostelium discoideum with a high affinity for poly(dA)-poly(dT). Nucleic Acids Res. 1983, 11: 8473-8484.PubMed CentralView ArticlePubMed
- Horn J, Dietz-Schmidt A, Zundorf I, Garin J, Dingermann T, Winckler T: A Dictyostelium protein binds to distinct oligo(dA)×oligo(dT) DNA sequences in the C-module of the retrotransposable element DRE. Eur J Biochem. 1999, 265: 441-448. 10.1046/j.1432-1327.1999.00768.x.View ArticlePubMed
- Moran LA, Scrimgeour KG, Horton HR, Ochs RS, Rawn JD: Biochemistry. Neil Patterson, Prentice Hall, Engelwood Cliffs, NJ. 1994, 2
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