Table 4 Bacterial strains and plasmids
Name | Relevant genotype/information a | Source/reference |
|---|---|---|
E. coli | ||
DH5αMCR | F- endA1 supE44 mcrA thi-1 hsdR17 λ- recA1 relA1 Δ (lacZYA-argF )U169 (Φ 80dlacZΔM15 ) gyrA96 deoR Δ (mrr-hsdRMS-mcrBC ) | [40] |
AB462, CGSC 462 | F- DE (gpt-proA )64 lacYl galK2 (Oc)hisG4 (Oc) λ- cysC9 xylA5 mtl-1 thi-1 | CGSCb |
JM226, CGSC 5468 | F- pro-0 lac -tsx -? galT47 trp-74 his -cysH57 xyl argA9999 rpsL -(strR) mal -ilvA -mtl - | CGSC, [47] |
JM221, CGSC 5745 | F- pro-50 lac- tsx -galT47 trp-74 his-97 cysD91 argA-rpsL--(strR) mat xyl-ilvA-mtl- | CGSC, [47] |
JM246, CGSC 5747 | F- λ- cysI53(Am)IN(rrnD-rrnE)1 rph-1 | CGSC, [47] |
C. glutamicum | ||
ATCC 13032 | Wild type, Nxr | ATCCc |
CR018 | Δcg3112 (cysZ) | this study |
CR019 | Δcg3113 (cysY) | this study |
CR020 | Δcg3114 (cysN) | this study |
CR021 | Δcg3115 (cysD) | this study |
CR022 | Δcg3116 (cysH) | this study |
CR023 | Δcg3117 (cysX) | this study |
CR024 | Δcg3118 (cysI) | this study |
CR025 | Δcg3119 (fpr2) | this study |
CR026 | cg3118::IS6100, pAT6100 | this study |
Plasmids | ||
pK18mobsacB | sacB, lacZa, Kmr, mcs mobilizable vector, allows for selection of double-crossover in C. glutamicum | [35] |
mobilizable vector, allows for selection of double-crossover in C. glutamicum | ||
pEC-XK99E | Ptrc, lacIq, Kmr inducible E. coli – C. glutamicum – shuttle expression vector | [48] |
pAT6100 | IS61 00, Kmr | [36] |
pCR018d | pK18mobsacB carrying cg3112 deld | this study |
pCR018e | pEC-XK99E carrying cysZ eve | this study |
pCR019d | pK18mobsacB carrying cg3113 del | this study |
pCR019e | pEC-XK99E carrying cysY ev | this study |
pCR020d | pK18mobsacB carrying cg3114 del | this study |
pCR020e | pEC-XK99E carrying cysDN ev | this study |
pCR021d | pK18mobsacB carrying cg3115 del | this study |
pCR022d | pK18mobsacB carrying cg3116 del | this study |
pCR022e | pEC-XK99E carrying cysH ev | this study |
pCR023d | pK18mobsacB carrying cg3117 del | this study |
pCR023e | pEC-XK99E carrying cysX ev | this study |
pCR024d | pK18mobsacB carrying cg3118 del | this study |
pCR024e | pEC-XK99E carrying cysI ev | this study |
pCR025d | pK18mobsacB carrying cg3119 del | this study |